The effects of low dose 60Co-gamma-radiation on radioresistance, mutagenesis, gene conversion, cell cycle and transcriptome profile in Saccharomyces cerevisiae
| العنوان: | The effects of low dose 60Co-gamma-radiation on radioresistance, mutagenesis, gene conversion, cell cycle and transcriptome profile in Saccharomyces cerevisiae |
|---|---|
| المؤلفون: | Madhu Bala, Meenakshi Dwivedi, Neeta Sehgal |
| المصدر: | International Journal of Low Radiation. 5:290 |
| بيانات النشر: | Inderscience Publishers, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Transcriptome, MRX complex, biology, Health, Toxicology and Mutagenesis, Radioresistance, Heat shock protein, Saccharomyces cerevisiae, Lethal dose, Public Health, Environmental and Occupational Health, Unfolded protein response, Cell cycle, biology.organism_classification, Molecular biology |
| الوصف: | The underlying principles of low (stress)-dose Radiation-Induced Radioresistance (RIR) are inadequately understood. The present investigation focuses on understanding the genetic basis of RIR in Saccharomyces cerevisiae. In comparison with non-pre-irradiated cultures, the cultures that were pre-irradiated with a low dose of 60Co γ-radiation showed increased survivors (32%) but decreased convertants (26%) and revertants (65%) after irradiation with a lethal dose of 60Co γ-radiation. The radioresistance was higher if the stress dose was delivered at lower dose rate. The prolonged S-phase upregulation of genes from clusters of signal transduction, DNA damage repair, stress response, cell cycle and energy generation and down-regulation of genes from Unfolded Protein Response (UPR) pathways were observed in stress-dose irradiated cells when studied with the microarray technique. The post-irradiation variations in Heat Shock Proteins (HSPs) and the Mre11-Rad50-Xrs2 (MRX) complex were time-dependent and confirmed by real-time Quantitative-Polymerase Chain Reaction (Q-PCR) and Western blotting. This study suggests a combined role of HSPs and the MRX complex in RIR. |
| تدمد: | 1741-9190 1477-6545 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e8ff3e0d25684636dc436bf25636510fTest https://doi.org/10.1504/ijlr.2008.020978Test |
| رقم الانضمام: | edsair.doi...........e8ff3e0d25684636dc436bf25636510f |
| قاعدة البيانات: | OpenAIRE |
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