Soluble Collagen VI treatment enhances mesenchymal stem cells expansion for engineering cartilage
العنوان: | Soluble Collagen VI treatment enhances mesenchymal stem cells expansion for engineering cartilage |
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المؤلفون: | Jieun Lee, Piera Smeriglio, Nidhi Bhutani |
المصدر: | Bioengineering & Translational Medicine. 2:278-284 |
بيانات النشر: | Wiley, 2017. |
سنة النشر: | 2017 |
مصطلحات موضوعية: | 030203 arthritis & rheumatology, 0301 basic medicine, Materials science, Cartilage, Mesenchymal stem cell, Biomedical Engineering, Pharmaceutical Science, Stem cell marker, Chondrogenesis, Cell biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Collagen VI, Immunology, medicine, Stem cell, Aggrecan, Biotechnology, Stem cell transplantation for articular cartilage repair |
الوصف: | Bone Marrow-derived mesenchymal stem cells (BM-MSC) are an attractive source for cell-based therapies in cartilage injury owing to their efficient differentiation into chondrocytes and their immune-suppressive abilities. However, their clinical use is hampered by a scarcity of cells leading to compromised efficacy. While expansion of human MSC ex vivo can potentially overcome the scarcity of cells, current methods lead to a rapid loss of the stem cell properties. In this study, we report soluble Collagen VI (cartilage pericellular matrix component) as a potential biologic that can expand the MSC population while maintaining the stem cell phenotype as confirmed by expression of the stem cell markers CD105 and CD90. Short-term treatment with Collagen VI additionally retains the potential of MSC to differentiate into mature chondrocytes in pellet culture. Cartilage pellets generated from MSC treated with Collagen VI or control express comparable amounts of the chondrogenic markers Collagen II, Aggrecan and Sox9, and the extracellular glycosaminoglycans. Our observations confirm that the use of the endogenous and cartilage-specific factor Collagen VI is valuable for a rapid and efficient expansion of MSC for potential use in cartilage regeneration and osteoarthritis. |
تدمد: | 2380-6761 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::4ef5b392d26dbb0911ece1e88c856e04Test https://doi.org/10.1002/btm2.10078Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi...........4ef5b392d26dbb0911ece1e88c856e04 |
قاعدة البيانات: | OpenAIRE |