دورية أكاديمية
Dynamic interplay between nitration and phosphorylation of tubulin cofactor B in the control of microtubule dynamics.
| العنوان: | Dynamic interplay between nitration and phosphorylation of tubulin cofactor B in the control of microtubule dynamics. |
|---|---|
| المؤلفون: | Rayala, Suresh K., Martin, Emil, Sharina, Iraida G., Molli, Poonam R., Xiaoping Wang, Jacobson, Raymond, Murad, Fend, Kumar, Rakesh |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America; 12/4/2007, Vol. 104 Issue 49, p19470-19475, 6p, 5 Graphs |
| مصطلحات موضوعية: | TUBULINS, EUKARYOTIC cells, PHOSPHORYLATION, CYTOSKELETON formation, NITRIC oxide, PROTEIN synthesis, PHYSIOLOGY |
| مستخلص: | Tubulin cofactor B (TCoB) plays an important role in microtubule dynamics by facilitating the dimerization of α- and β-tubulin. Recent evidence suggests that p21-activated kinase 1 (Pak1), a major signaling nodule in eukaryotic cells. phosphorylates TCoB on Ser-65 and Ser-128 and plays an essential role in microtubule regrowth. However, to date, no upstream signaling molecules have been identified to antagonize the functions of TCoB, which might help in maintaining the equilibrium of microtubules. Here, we discovered that TCoB is efficiently nitrated, mainly on Tyr-64 and Tyr-98, and nitrated-TCoB attenuates the synthesis of new microtubules. In addition, we found that nitration of TCoB antagonizes signaling-dependent phosphorylation of TCoB, whereas optimal nitration of TCoB requires the presence of functional Paki phosphorylation sites, thus providing a feedback mechanism to regulate phosphorylation-dependent MT regrowth. Together these findings identified TCoB as the third cytoskeleton protein to be nitrated and suggest a previously undescribed mechanism, whereby growth factor signaling may coordinately integrate nitric oxide signaling in the regulation of microtubule dynamics. [ABSTRACT FROM AUTHOR] |
| Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
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