دورية أكاديمية
Distinct Injury Responsive Regulatory T Cells Identified by Multi-Dimensional Phenotyping.
| العنوان: | Distinct Injury Responsive Regulatory T Cells Identified by Multi-Dimensional Phenotyping. |
|---|---|
| المؤلفون: | Guo, Fei, Hancock, Brandon, Griffith, Alec, Lin, Hui, Howard, Kaitlyn, Keegan, Joshua, Zhang, Fan, Chicoine, Adam, Cahill, Laura, Ng, Julie, Lederer, James |
| المصدر: | Frontiers in Immunology; 5/12/2022, Vol. 13, p1-17, 17p |
| مصطلحات موضوعية: | REGULATORY T cells, T cell receptors, RNA sequencing, CHEMOKINE receptors, CELL receptors |
| مستخلص: | CD4+ regulatory T cells (Tregs) activate and expand in response to different types of injuries, suggesting that they play a critical role in controlling the immune response to tissue and cell damage. This project used multi-dimensional profiling techniques to comprehensively characterize injury responsive Tregs in mice. We show that CD44high Tregs expand in response to injury and were highly suppressive when compared to CD44low Tregs. T cell receptor (TCR) repertoire analysis revealed that the CD44high Treg population undergo TCRαβ clonal expansion as well as increased TCR CDR3 diversity. Bulk RNA sequencing and single-cell RNA sequencing with paired TCR clonotype analysis identified unique differences between CD44high and CD44low Tregs and specific upregulation of genes in Tregs with expanded TCR clonotypes. Gene ontology analysis for molecular function of RNA sequencing data identified chemokine receptors and cell division as the most enriched functional terms in CD44high Tregs versus CD44low Tregs. Mass cytometry (CyTOF) analysis of Tregs from injured and uninjured mice verified protein expression of these genes on CD44high Tregs, with injury-induced increases in Helios, Galectin-3 and PYCARD expression. Taken together, these data indicate that injury triggers the expansion of a highly suppressive CD44high Treg population that is transcriptionally and phenotypically distinct from CD44low Tregs suggesting that they actively participate in controlling immune responses to injury and tissue damage. [ABSTRACT FROM AUTHOR] |
| Copyright of Frontiers in Immunology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edb Complementary Index 156844880 965 6 Academic Journal academicJournal 965.135437011719 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edb&AN=156844880&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edb&genre=article&issn=16643224&ISBN=&volume=13&issue=&date=20220512&spage=1&pages=1-17&title=Frontiers in Immunology&atitle=Distinct%20Injury%20Responsive%20Regulatory%20T%20Cells%20Identified%20by%20Multi-Dimensional%20Phenotyping.&id=DOI:10.3389/fimmu.2022.833100 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Distinct Injury Responsive Regulatory T Cells Identified by Multi-Dimensional Phenotyping.
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Guo%2C+Fei%22">Guo, Fei</searchLink><br /><searchLink fieldCode="AR" term="%22Hancock%2C+Brandon%22">Hancock, Brandon</searchLink><br /><searchLink fieldCode="AR" term="%22Griffith%2C+Alec%22">Griffith, Alec</searchLink><br /><searchLink fieldCode="AR" term="%22Lin%2C+Hui%22">Lin, Hui</searchLink><br /><searchLink fieldCode="AR" term="%22Howard%2C+Kaitlyn%22">Howard, Kaitlyn</searchLink><br /><searchLink fieldCode="AR" term="%22Keegan%2C+Joshua%22">Keegan, Joshua</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Fan%22">Zhang, Fan</searchLink><br /><searchLink fieldCode="AR" term="%22Chicoine%2C+Adam%22">Chicoine, Adam</searchLink><br /><searchLink fieldCode="AR" term="%22Cahill%2C+Laura%22">Cahill, Laura</searchLink><br /><searchLink fieldCode="AR" term="%22Ng%2C+Julie%22">Ng, Julie</searchLink><br /><searchLink fieldCode="AR" term="%22Lederer%2C+James%22">Lederer, James</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Frontiers in Immunology; 5/12/2022, Vol. 13, p1-17, 17p ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22REGULATORY+T+cells%22">REGULATORY T cells</searchLink><br /><searchLink fieldCode="DE" term="%22T+cell+receptors%22">T cell receptors</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22CHEMOKINE+receptors%22">CHEMOKINE receptors</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+receptors%22">CELL receptors</searchLink> ) Array ( [Name] => Abstract [Label] => Abstract [Group] => Ab [Data] => CD4<superscript>+</superscript> regulatory T cells (Tregs) activate and expand in response to different types of injuries, suggesting that they play a critical role in controlling the immune response to tissue and cell damage. This project used multi-dimensional profiling techniques to comprehensively characterize injury responsive Tregs in mice. We show that CD44<superscript>high</superscript> Tregs expand in response to injury and were highly suppressive when compared to CD44<superscript>low</superscript> Tregs. T cell receptor (TCR) repertoire analysis revealed that the CD44<superscript>high</superscript> Treg population undergo TCRαβ clonal expansion as well as increased TCR CDR3 diversity. Bulk RNA sequencing and single-cell RNA sequencing with paired TCR clonotype analysis identified unique differences between CD44<superscript>high</superscript> and CD44<superscript>low</superscript> Tregs and specific upregulation of genes in Tregs with expanded TCR clonotypes. Gene ontology analysis for molecular function of RNA sequencing data identified chemokine receptors and cell division as the most enriched functional terms in CD44<superscript>high</superscript> Tregs versus CD44<superscript>low</superscript> Tregs. Mass cytometry (CyTOF) analysis of Tregs from injured and uninjured mice verified protein expression of these genes on CD44<superscript>high</superscript> Tregs, with injury-induced increases in Helios, Galectin-3 and PYCARD expression. Taken together, these data indicate that injury triggers the expansion of a highly suppressive CD44<superscript>high</superscript> Treg population that is transcriptionally and phenotypically distinct from CD44<superscript>low</superscript> Tregs suggesting that they actively participate in controlling immune responses to injury and tissue damage. [ABSTRACT FROM AUTHOR] ) Array ( [Name] => Abstract [Label] => [Group] => Ab [Data] => <i>Copyright of Frontiers in Immunology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3389/fimmu.2022.833100
)
)
[Languages] => Array
(
[0] => Array
(
[Code] => eng
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 17
[StartPage] => 1
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => REGULATORY T cells
[Type] => general
)
[1] => Array
(
[SubjectFull] => T cell receptors
[Type] => general
)
[2] => Array
(
[SubjectFull] => RNA sequencing
[Type] => general
)
[3] => Array
(
[SubjectFull] => CHEMOKINE receptors
[Type] => general
)
[4] => Array
(
[SubjectFull] => CELL receptors
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Distinct Injury Responsive Regulatory T Cells Identified by Multi-Dimensional Phenotyping.
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Guo, Fei
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Hancock, Brandon
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Griffith, Alec
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lin, Hui
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Howard, Kaitlyn
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Keegan, Joshua
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Zhang, Fan
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Chicoine, Adam
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Cahill, Laura
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ng, Julie
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lederer, James
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 12
[M] => 05
[Text] => 5/12/2022
[Type] => published
[Y] => 2022
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 16643224
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 13
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Frontiers in Immunology
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |