دورية أكاديمية
Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells.
| العنوان: | Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells. |
|---|---|
| المؤلفون: | Traenkle, Bjoern, Kaiser, Philipp D., Pezzana, Stefania, Richardson, Jennifer, Gramlich, Marius, Wagner, Teresa R., Seyfried, Dominik, Weldle, Melissa, Holz, Stefanie, Parfyonova, Yana, Nueske, Stefan, Scholz, Armin M., Zeck, Anne, Jakobi, Meike, Schneiderhan-Marra, Nicole, Schaller, Martin, Maurer, Andreas, Gouttefangeas, Cécile, Kneilling, Manfred, Pichler, Bernd J. |
| المصدر: | Frontiers in Immunology; 12/9/2021, Vol. 12, p1-17, 17p |
| مصطلحات موضوعية: | POSITRON emission tomography, MAGNETIC resonance imaging, IMMUNOGLOBULINS, LABORATORY mice, CD4 antigen, MOLECULAR probes |
| مستخلص: | The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4+ cells in regulating immunological processes, we generated novel single-domain antibodies [nanobodies (Nbs)] that specifically recognize human CD4. After in-depth analysis of their binding properties, recognized epitopes, and effects on T-cell proliferation, activation, and cytokine release, we selected CD4-specific Nbs that did not interfere with crucial T-cell processes in vitro and converted them into immune tracers for noninvasive molecular imaging. By optical imaging, we demonstrated the ability of a high-affinity CD4-Nb to specifically visualize CD4+ cells in vivo using a xenograft model. Furthermore, quantitative high-resolution immune positron emission tomography (immunoPET)/MR of a human CD4 knock-in mouse model showed rapid accumulation of 64Cu-radiolabeled CD4-Nb1 in CD4+ T cell-rich tissues. We propose that the CD4-Nbs presented here could serve as versatile probes for stratifying patients and monitoring individual immune responses during personalized immunotherapy in both cancer and inflammatory diseases. [ABSTRACT FROM AUTHOR] |
| Copyright of Frontiers in Immunology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edb Complementary Index 154043991 949 6 Academic Journal academicJournal 949.122436523438 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edb&AN=154043991&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edb&genre=article&issn=16643224&ISBN=&volume=12&issue=&date=20211209&spage=1&pages=1-17&title=Frontiers in Immunology&atitle=Single-Domain%20Antibodies%20for%20Targeting%2C%20Detection%2C%20and%20In%20Vivo%20Imaging%20of%20Human%20CD4%2B%20Cells.&id=DOI:10.3389/fimmu.2021.799910 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4<superscript>+</superscript> Cells.
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Traenkle%2C+Bjoern%22">Traenkle, Bjoern</searchLink><br /><searchLink fieldCode="AR" term="%22Kaiser%2C+Philipp+D%2E%22">Kaiser, Philipp D.</searchLink><br /><searchLink fieldCode="AR" term="%22Pezzana%2C+Stefania%22">Pezzana, Stefania</searchLink><br /><searchLink fieldCode="AR" term="%22Richardson%2C+Jennifer%22">Richardson, Jennifer</searchLink><br /><searchLink fieldCode="AR" term="%22Gramlich%2C+Marius%22">Gramlich, Marius</searchLink><br /><searchLink fieldCode="AR" term="%22Wagner%2C+Teresa+R%2E%22">Wagner, Teresa R.</searchLink><br /><searchLink fieldCode="AR" term="%22Seyfried%2C+Dominik%22">Seyfried, Dominik</searchLink><br /><searchLink fieldCode="AR" term="%22Weldle%2C+Melissa%22">Weldle, Melissa</searchLink><br /><searchLink fieldCode="AR" term="%22Holz%2C+Stefanie%22">Holz, Stefanie</searchLink><br /><searchLink fieldCode="AR" term="%22Parfyonova%2C+Yana%22">Parfyonova, Yana</searchLink><br /><searchLink fieldCode="AR" term="%22Nueske%2C+Stefan%22">Nueske, Stefan</searchLink><br /><searchLink fieldCode="AR" term="%22Scholz%2C+Armin+M%2E%22">Scholz, Armin M.</searchLink><br /><searchLink fieldCode="AR" term="%22Zeck%2C+Anne%22">Zeck, Anne</searchLink><br /><searchLink fieldCode="AR" term="%22Jakobi%2C+Meike%22">Jakobi, Meike</searchLink><br /><searchLink fieldCode="AR" term="%22Schneiderhan-Marra%2C+Nicole%22">Schneiderhan-Marra, Nicole</searchLink><br /><searchLink fieldCode="AR" term="%22Schaller%2C+Martin%22">Schaller, Martin</searchLink><br /><searchLink fieldCode="AR" term="%22Maurer%2C+Andreas%22">Maurer, Andreas</searchLink><br /><searchLink fieldCode="AR" term="%22Gouttefangeas%2C+Cécile%22">Gouttefangeas, Cécile</searchLink><br /><searchLink fieldCode="AR" term="%22Kneilling%2C+Manfred%22">Kneilling, Manfred</searchLink><br /><searchLink fieldCode="AR" term="%22Pichler%2C+Bernd+J%2E%22">Pichler, Bernd J.</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Frontiers in Immunology; 12/9/2021, Vol. 12, p1-17, 17p ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22POSITRON+emission+tomography%22">POSITRON emission tomography</searchLink><br /><searchLink fieldCode="DE" term="%22MAGNETIC+resonance+imaging%22">MAGNETIC resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNOGLOBULINS%22">IMMUNOGLOBULINS</searchLink><br /><searchLink fieldCode="DE" term="%22LABORATORY+mice%22">LABORATORY mice</searchLink><br /><searchLink fieldCode="DE" term="%22CD4+antigen%22">CD4 antigen</searchLink><br /><searchLink fieldCode="DE" term="%22MOLECULAR+probes%22">MOLECULAR probes</searchLink> ) Array ( [Name] => Abstract [Label] => Abstract [Group] => Ab [Data] => The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4<superscript>+</superscript> cells in regulating immunological processes, we generated novel single-domain antibodies [nanobodies (Nbs)] that specifically recognize human CD4. After in-depth analysis of their binding properties, recognized epitopes, and effects on T-cell proliferation, activation, and cytokine release, we selected CD4-specific Nbs that did not interfere with crucial T-cell processes in vitro and converted them into immune tracers for noninvasive molecular imaging. By optical imaging, we demonstrated the ability of a high-affinity CD4-Nb to specifically visualize CD4<superscript>+</superscript> cells in vivo using a xenograft model. Furthermore, quantitative high-resolution immune positron emission tomography (immunoPET)/MR of a human CD4 knock-in mouse model showed rapid accumulation of <superscript>64</superscript>Cu-radiolabeled CD4-Nb1 in CD4<superscript>+</superscript> T cell-rich tissues. We propose that the CD4-Nbs presented here could serve as versatile probes for stratifying patients and monitoring individual immune responses during personalized immunotherapy in both cancer and inflammatory diseases. [ABSTRACT FROM AUTHOR] ) Array ( [Name] => Abstract [Label] => [Group] => Ab [Data] => <i>Copyright of Frontiers in Immunology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3389/fimmu.2021.799910
)
)
[Languages] => Array
(
[0] => Array
(
[Code] => eng
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 17
[StartPage] => 1
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => POSITRON emission tomography
[Type] => general
)
[1] => Array
(
[SubjectFull] => MAGNETIC resonance imaging
[Type] => general
)
[2] => Array
(
[SubjectFull] => IMMUNOGLOBULINS
[Type] => general
)
[3] => Array
(
[SubjectFull] => LABORATORY mice
[Type] => general
)
[4] => Array
(
[SubjectFull] => CD4 antigen
[Type] => general
)
[5] => Array
(
[SubjectFull] => MOLECULAR probes
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells.
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Traenkle, Bjoern
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Kaiser, Philipp D.
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Pezzana, Stefania
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Richardson, Jennifer
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gramlich, Marius
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Wagner, Teresa R.
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Seyfried, Dominik
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Weldle, Melissa
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Holz, Stefanie
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Parfyonova, Yana
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Nueske, Stefan
)
)
)
[11] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Scholz, Armin M.
)
)
)
[12] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Zeck, Anne
)
)
)
[13] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jakobi, Meike
)
)
)
[14] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Schneiderhan-Marra, Nicole
)
)
)
[15] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Schaller, Martin
)
)
)
[16] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Maurer, Andreas
)
)
)
[17] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gouttefangeas, Cécile
)
)
)
[18] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Kneilling, Manfred
)
)
)
[19] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Pichler, Bernd J.
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 09
[M] => 12
[Text] => 12/9/2021
[Type] => published
[Y] => 2021
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 16643224
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 12
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Frontiers in Immunology
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |