دورية أكاديمية
Limitations of a Commercial Assay as Diagnostic Test of Autoimmune Encephalitis.
العنوان: | Limitations of a Commercial Assay as Diagnostic Test of Autoimmune Encephalitis. |
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المؤلفون: | Ruiz-García, Raquel, Muñoz-Sánchez, Guillermo, Naranjo, Laura, Guasp, Mar, Sabater, Lidia, Saiz, Albert, Dalmau, Josep, Graus, Francesc, Martinez-Hernandez, Eugenia |
المصدر: | Frontiers in Immunology; 6/29/2021, Vol. 12, p1-8, 8p |
مصطلحات موضوعية: | DIAGNOSIS methods, ENCEPHALITIS, DIAGNOSTIC reagents & test kits, DIAGNOSIS, IMMUNOFLUORESCENCE |
مصطلحات جغرافية: | LUBECK (Germany) |
مستخلص: | Detection of neuronal surface antibodies (NSAb) is important for the diagnosis of autoimmune encephalitis (AE). Although most clinical laboratories use a commercial diagnostic kit (Euroimmun, Lübeck, Germany) based on indirect immunofluorescence on transfected cells (IIFA), clinical experience suggests diagnostic limitations. Here, we assessed the performance of the commercial IIFA in serum and CSF samples of patients with suspected AE previously examined by rat brain immunohistochemistry (Cohort A). Of 6213 samples, 404 (6.5%) showed brain immunostaining suggestive of NSAb: 163 (40%) were positive by commercial IIFA and 241 (60%) were negative. When these 241 samples were re-assessed with in-house IIFA, 42 (18%) were positive: 21 (9%) had NSAb against antigens not included in the commercial IIFA and the other 21 (9%) had NSAb against antigens included in the commercial kit (false negative results). False negative results occurred more frequently with CSF (29% vs 10% in serum) and predominantly affected GABA |
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قاعدة البيانات: | Complementary Index |
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