دورية أكاديمية
Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice.
| العنوان: | Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice. |
|---|---|
| المؤلفون: | Godakova, Svetlana A., Noskov, Anatoly N., Vinogradova, Irina D., Ugriumova, Galina A., Solovyev, Andrey I., Esmagambetov, Ilias B., Tukhvatulin, Amir I., Logunov, Denis Y., Naroditsky, Boris S., Shcheblyakov, Dmitry V., Gintsburg, Aleksandr L. |
| المصدر: | Toxins; Aug2019, Vol. 11 Issue 8, p464-464, 1p |
| مصطلحات موضوعية: | BOTULINUM A toxins, CLOSTRIDIUM botulinum, BOTULINUM toxin, NERVOUS system, BOTULISM, IMMUNOGLOBULIN G, MICE |
| مستخلص: | The bacterium Clostridium botulinum is the causative agent of botulism—a severe intoxication caused by botulinum neurotoxin (BoNT) and characterized by damage to the nervous system. In an effort to develop novel C. botulinum immunotherapeutics, camelid single-domain antibodies (sdAbs, VHHs, or nanobodies) could be used due to their unique structure and characteristics. In this study, VHHs were produced using phage display technology. A total of 15 different monoclonal VHHs were selected based on their comlementarity-determining region 3 (CDR3) sequences. Different toxin lethal dose (LD |
| Copyright of Toxins is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edb Complementary Index 138176313 805 6 Academic Journal academicJournal 805.07666015625 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edb&AN=138176313&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edb&genre=article&issn=20726651&ISBN=&volume=11&issue=8&date=20190801&spage=464&pages=464-464&title=Toxins&atitle=Camelid%20VHHs%20Fused%20to%20Human%20Fc%20Fragments%20Provide%20Long%20Term%20Protection%20Against%20Botulinum%20Neurotoxin%20A%20in%20Mice.&id=DOI:10.3390/toxins11080464 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice.
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Godakova%2C+Svetlana+A%2E%22">Godakova, Svetlana A.</searchLink><br /><searchLink fieldCode="AR" term="%22Noskov%2C+Anatoly+N%2E%22">Noskov, Anatoly N.</searchLink><br /><searchLink fieldCode="AR" term="%22Vinogradova%2C+Irina+D%2E%22">Vinogradova, Irina D.</searchLink><br /><searchLink fieldCode="AR" term="%22Ugriumova%2C+Galina+A%2E%22">Ugriumova, Galina A.</searchLink><br /><searchLink fieldCode="AR" term="%22Solovyev%2C+Andrey+I%2E%22">Solovyev, Andrey I.</searchLink><br /><searchLink fieldCode="AR" term="%22Esmagambetov%2C+Ilias+B%2E%22">Esmagambetov, Ilias B.</searchLink><br /><searchLink fieldCode="AR" term="%22Tukhvatulin%2C+Amir+I%2E%22">Tukhvatulin, Amir I.</searchLink><br /><searchLink fieldCode="AR" term="%22Logunov%2C+Denis+Y%2E%22">Logunov, Denis Y.</searchLink><br /><searchLink fieldCode="AR" term="%22Naroditsky%2C+Boris+S%2E%22">Naroditsky, Boris S.</searchLink><br /><searchLink fieldCode="AR" term="%22Shcheblyakov%2C+Dmitry+V%2E%22">Shcheblyakov, Dmitry V.</searchLink><br /><searchLink fieldCode="AR" term="%22Gintsburg%2C+Aleksandr+L%2E%22">Gintsburg, Aleksandr L.</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Toxins; Aug2019, Vol. 11 Issue 8, p464-464, 1p ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22BOTULINUM+A+toxins%22">BOTULINUM A toxins</searchLink><br /><searchLink fieldCode="DE" term="%22CLOSTRIDIUM+botulinum%22">CLOSTRIDIUM botulinum</searchLink><br /><searchLink fieldCode="DE" term="%22BOTULINUM+toxin%22">BOTULINUM toxin</searchLink><br /><searchLink fieldCode="DE" term="%22NERVOUS+system%22">NERVOUS system</searchLink><br /><searchLink fieldCode="DE" term="%22BOTULISM%22">BOTULISM</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNOGLOBULIN+G%22">IMMUNOGLOBULIN G</searchLink><br /><searchLink fieldCode="DE" term="%22MICE%22">MICE</searchLink> ) Array ( [Name] => Abstract [Label] => Abstract [Group] => Ab [Data] => The bacterium Clostridium botulinum is the causative agent of botulism—a severe intoxication caused by botulinum neurotoxin (BoNT) and characterized by damage to the nervous system. In an effort to develop novel C. botulinum immunotherapeutics, camelid single-domain antibodies (sdAbs, VHHs, or nanobodies) could be used due to their unique structure and characteristics. In this study, VHHs were produced using phage display technology. A total of 15 different monoclonal VHHs were selected based on their comlementarity-determining region 3 (CDR3) sequences. Different toxin lethal dose (LD<subscript>50</subscript>) challenges with each selected phage clone were conducted in vivo to check their neutralizing potency. We demonstrated that modification of neutralizing VHHs with a human immunoglobulin G (IgG)1 Fc (fragment crystallizable) fragment (fusionbody, VHH-Fc) significantly increased the circulation time in the blood (up to 14 days). At the same time, VHH-Fc showed the protective activity 1000 times higher than monomeric form when challenged with 5 LD<subscript>50</subscript>. Moreover, VHH-Fcs remained protective even 14 days after antibody administration. These results indicate that this VHH-Fc could be used as an effective long term antitoxin protection against botulinum type A. [ABSTRACT FROM AUTHOR] ) Array ( [Name] => Abstract [Label] => [Group] => Ab [Data] => <i>Copyright of Toxins is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3390/toxins11080464
)
)
[Languages] => Array
(
[0] => Array
(
[Code] => eng
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 1
[StartPage] => 464
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => BOTULINUM A toxins
[Type] => general
)
[1] => Array
(
[SubjectFull] => CLOSTRIDIUM botulinum
[Type] => general
)
[2] => Array
(
[SubjectFull] => BOTULINUM toxin
[Type] => general
)
[3] => Array
(
[SubjectFull] => NERVOUS system
[Type] => general
)
[4] => Array
(
[SubjectFull] => BOTULISM
[Type] => general
)
[5] => Array
(
[SubjectFull] => IMMUNOGLOBULIN G
[Type] => general
)
[6] => Array
(
[SubjectFull] => MICE
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice.
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Godakova, Svetlana A.
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Noskov, Anatoly N.
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Vinogradova, Irina D.
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ugriumova, Galina A.
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Solovyev, Andrey I.
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Esmagambetov, Ilias B.
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Tukhvatulin, Amir I.
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Logunov, Denis Y.
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Naroditsky, Boris S.
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Shcheblyakov, Dmitry V.
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gintsburg, Aleksandr L.
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 08
[Text] => Aug2019
[Type] => published
[Y] => 2019
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 20726651
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 11
)
[1] => Array
(
[Type] => issue
[Value] => 8
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Toxins
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |