دورية أكاديمية
Stimulation of HERG Channel Activity by β-catenin.
| العنوان: | Stimulation of HERG Channel Activity by β-catenin. |
|---|---|
| المؤلفون: | Munoz, Carlos1, Saxena, Ambrish1, Pakladok, Tatsiana1, Bogatikov, Evgenii1, Wilmes, Jan1, Seebohm, Guiscard2, Föller, Michael1,3, Lang, Florian1 florian.lang@uni-tuebingen.de, Uversky, Vladimir N.4 |
| المصدر: | PLoS ONE. Aug2012, Vol. 7 Issue 8, Special section p1-5. 5p. |
| مصطلحات موضوعية: | *GENE expression, *CELL proliferation, *CELL membranes, *XENOPUS eggs, *ACTINOMYCIN, *BREFELDIN |
| مستخلص: | The multifunctional protein β-catenin governs as transcription factor the expression of a wide variety of genes relevant for cell proliferation and cell survival. In addition, β-catenin is localized at the cell membrane and may influence the function of channels. The present study explored the possibility that beta;-catenin participates in the regulation of the HERG K+ channel. To this end, HERG was expressed in Xenopus oocytes with or without β-catenin and the voltage-gated current determined utilizing the dual electrode voltage clamp. As a result, expression of β-catenin markedly upregulated HERG channel activity, an effect not sensitive to inhibition of transcription with actinomycin D (10 µM). According to chemiluminescence, β-catenin may increase HERG channel abundance within the oocyte cell membrane. Following inhibition of channel insertion into the cell membrane by brefeldin A (5 mM) the decay of current was similar in oocytes expressing HERG together with β-catenin to oocytes expressing HERG alone. The experiments uncover a novel function of APC/β-catenin, i.e. the regulation of HERG channels. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: | Academic Search Index |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
asx Academic Search Index 80435071 1206 6 Academic Journal academicJournal 1206.3115234375 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=asx&AN=80435071&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:asx&genre=article&issn=19326203&ISBN=&volume=7&issue=8&date=20120801&spage=1&pages=1-5&title=PLoS ONE&atitle=Stimulation%20of%20HERG%20Channel%20Activity%20by%20%CE%B2-catenin.&id=DOI:10.1371/journal.pone.0043353 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Stimulation of HERG Channel Activity by β-catenin.
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Munoz%2C+Carlos%22">Munoz, Carlos</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Saxena%2C+Ambrish%22">Saxena, Ambrish</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pakladok%2C+Tatsiana%22">Pakladok, Tatsiana</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bogatikov%2C+Evgenii%22">Bogatikov, Evgenii</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wilmes%2C+Jan%22">Wilmes, Jan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Seebohm%2C+Guiscard%22">Seebohm, Guiscard</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Föller%2C+Michael%22">Föller, Michael</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Lang%2C+Florian%22">Lang, Florian</searchLink><relatesTo>1</relatesTo><i> florian.lang@uni-tuebingen.de</i><br /><searchLink fieldCode="AR" term="%22Uversky%2C+Vladimir+N%2E%22">Uversky, Vladimir N.</searchLink><relatesTo>4</relatesTo> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <searchLink fieldCode="JN" term="%22PLoS+ONE%22">PLoS ONE</searchLink>. Aug2012, Vol. 7 Issue 8, Special section p1-5. 5p. ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => *<searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+proliferation%22">CELL proliferation</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+membranes%22">CELL membranes</searchLink><br />*<searchLink fieldCode="DE" term="%22XENOPUS+eggs%22">XENOPUS eggs</searchLink><br />*<searchLink fieldCode="DE" term="%22ACTINOMYCIN%22">ACTINOMYCIN</searchLink><br />*<searchLink fieldCode="DE" term="%22BREFELDIN%22">BREFELDIN</searchLink> ) Array ( [Name] => Abstract [Label] => Abstract [Group] => Ab [Data] => The multifunctional protein β-catenin governs as transcription factor the expression of a wide variety of genes relevant for cell proliferation and cell survival. In addition, β-catenin is localized at the cell membrane and may influence the function of channels. The present study explored the possibility that beta;-catenin participates in the regulation of the HERG K+ channel. To this end, HERG was expressed in Xenopus oocytes with or without β-catenin and the voltage-gated current determined utilizing the dual electrode voltage clamp. As a result, expression of β-catenin markedly upregulated HERG channel activity, an effect not sensitive to inhibition of transcription with actinomycin D (10 µM). According to chemiluminescence, β-catenin may increase HERG channel abundance within the oocyte cell membrane. Following inhibition of channel insertion into the cell membrane by brefeldin A (5 mM) the decay of current was similar in oocytes expressing HERG together with β-catenin to oocytes expressing HERG alone. The experiments uncover a novel function of APC/β-catenin, i.e. the regulation of HERG channels. [ABSTRACT FROM AUTHOR] ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1371/journal.pone.0043353
)
)
[Languages] => Array
(
[0] => Array
(
[Code] => eng
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 5
[StartPage] => 1
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => GENE expression
[Type] => general
)
[1] => Array
(
[SubjectFull] => CELL proliferation
[Type] => general
)
[2] => Array
(
[SubjectFull] => CELL membranes
[Type] => general
)
[3] => Array
(
[SubjectFull] => XENOPUS eggs
[Type] => general
)
[4] => Array
(
[SubjectFull] => ACTINOMYCIN
[Type] => general
)
[5] => Array
(
[SubjectFull] => BREFELDIN
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Stimulation of HERG Channel Activity by β-catenin.
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Munoz, Carlos
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Saxena, Ambrish
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Pakladok, Tatsiana
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Bogatikov, Evgenii
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Wilmes, Jan
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Seebohm, Guiscard
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Föller, Michael
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lang, Florian
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Uversky, Vladimir N.
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 08
[Text] => Aug2012
[Type] => published
[Y] => 2012
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 19326203
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 7
)
[1] => Array
(
[Type] => issue
[Value] => 8
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => PLoS ONE
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |