دورية أكاديمية
Experimental application of the median-effect principle for in vitro quantification of the combined inhibitory activities of clavulanic acid and imipenem against IRT-4 β-lactamase
| العنوان: | Experimental application of the median-effect principle for in vitro quantification of the combined inhibitory activities of clavulanic acid and imipenem against IRT-4 β-lactamase |
|---|---|
| المؤلفون: | Sotto, Albert1 albert.sotto@chu-nimes.fr, Foulongne, Vincent2, Sirot, Danielle3, Labia, Roger4, Jourdan, Jacques1 |
| المصدر: | International Journal of Antimicrobial Agents. Jan2002, Vol. 19 Issue 1, p75. 4p. |
| مصطلحات موضوعية: | *BETA lactamases, *ENTEROBACTERIACEAE, *CEPHALOSPORINS |
| مستخلص: | Inhibitor-resistant TEM β-lactamases (IRT) have been identified in Enterobacteriaceae which, however, remained susceptible to cephalosporins. We evaluated the combined inhibitory activity of clavulanic acid and imipenem at ratios of 1:1 and 1:3 against IRT-4, using the median effect principle of Chou and Talalay. The combination of the two drugs, which produced a nearly additive effect, meant their concentrations could be lowered 1.3–4.9-fold, while maintaining a 50% inhibitory effect against the IRT-4 in comparison with each drug alone. From a therapeutic point of view, such a combination is not efficient but this method of Chou and Talalay, used for the first time to assay combined inhibitory activity of β-lactamase inhibitors, could be used to evaluate new molecules and/or strategies to inactivate β-lactamase. [Copyright &y& Elsevier] |
| قاعدة البيانات: | Academic Search Index |
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