دورية أكاديمية
Influence of carbon nanotube scaffolds on human cervical carcinoma HeLa cell viability and focal adhesion kinase expression
العنوان: | Influence of carbon nanotube scaffolds on human cervical carcinoma HeLa cell viability and focal adhesion kinase expression |
---|---|
المؤلفون: | Zhang, Xiaoke1, Wang, Xuefeng1,2, Lu, Qinghua1 qhlu@sjtu.edu.cn, Fu, Chuanlong1 |
المصدر: | Carbon. Mar2008, Vol. 46 Issue 3, p453-460. 8p. |
مصطلحات موضوعية: | *CARBON nanotubes, *VACUUM, *CERVICAL cancer, *FOCAL adhesion kinase, *CELL culture |
مستخلص: | Abstract: Five types of carbon nanotube (CNT) scaffolds were prepared by vacuum filtration of the dispersed single-wall carbon nanotubes (SWCNTs), acid-treated SWCNTs, multi-wall carbon nanotubes (MWCNTs), acid-treated MWCNTs, and amylose-wrapped SWCNTs in water onto porous poly(vinylidene chloride) membranes. The influence of these scaffolds on human cervical carcinoma HeLa cells was investigated by WST-1 assay, acridine orange/ethidium bromide double staining and 1,1′-dioctadecyl-3,3,3′,3′tetram-ethylindocarbocyanine perchlorate staining. The results indicated that the viability of HeLa cells cultured on these scaffolds decreased in the following order: amylose-wrapped SWCNTs>acid-treated MWCNTs>MWCNTs>acid-treated SWCNTs>SWCNTs. Cells cultured on SWCNTs and on acid-treated SWCNTs were found undergoing apoptosis with damaged cell membrane and condensed chromatin. The result of an immunocytochemical test showed that both “dot-like” and “dash-like” focal adhesion kinases (FAKs) mainly distributed at the periphery of cells cultured on SWCNTs, while “dot-like” FAKs distributed in the whole cell body of cells cultured on MWCNTs. We therefore hypothesize that FAK expression might play a key role in controlling cell viability for cells cultured on CNT scaffolds. [Copyright &y& Elsevier] |
قاعدة البيانات: | Academic Search Index |
ResultId |
1 |
---|---|
Header |
asx Academic Search Index 31306482 1203 6 Academic Journal academicJournal 1203.18957519531 |
PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=asx&AN=31306482&custid=s6537998&authtype=sso |
FullText |
Array
(
[Availability] => 0
)
|
Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Influence of carbon nanotube scaffolds on human cervical carcinoma HeLa cell viability and focal adhesion kinase expression
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Zhang%2C+Xiaoke%22">Zhang, Xiaoke</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Xuefeng%22">Wang, Xuefeng</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lu%2C+Qinghua%22">Lu, Qinghua</searchLink><relatesTo>1</relatesTo><i> qhlu@sjtu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Fu%2C+Chuanlong%22">Fu, Chuanlong</searchLink><relatesTo>1</relatesTo> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <searchLink fieldCode="JN" term="%22Carbon%22">Carbon</searchLink>. Mar2008, Vol. 46 Issue 3, p453-460. 8p. ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => *<searchLink fieldCode="DE" term="%22CARBON+nanotubes%22">CARBON nanotubes</searchLink><br />*<searchLink fieldCode="DE" term="%22VACUUM%22">VACUUM</searchLink><br />*<searchLink fieldCode="DE" term="%22CERVICAL+cancer%22">CERVICAL cancer</searchLink><br />*<searchLink fieldCode="DE" term="%22FOCAL+adhesion+kinase%22">FOCAL adhesion kinase</searchLink><br />*<searchLink fieldCode="DE" term="%22CELL+culture%22">CELL culture</searchLink> ) Array ( [Name] => Abstract [Label] => Abstract [Group] => Ab [Data] => Abstract: Five types of carbon nanotube (CNT) scaffolds were prepared by vacuum filtration of the dispersed single-wall carbon nanotubes (SWCNTs), acid-treated SWCNTs, multi-wall carbon nanotubes (MWCNTs), acid-treated MWCNTs, and amylose-wrapped SWCNTs in water onto porous poly(vinylidene chloride) membranes. The influence of these scaffolds on human cervical carcinoma HeLa cells was investigated by WST-1 assay, acridine orange/ethidium bromide double staining and 1,1′-dioctadecyl-3,3,3′,3′tetram-ethylindocarbocyanine perchlorate staining. The results indicated that the viability of HeLa cells cultured on these scaffolds decreased in the following order: amylose-wrapped SWCNTs>acid-treated MWCNTs>MWCNTs>acid-treated SWCNTs>SWCNTs. Cells cultured on SWCNTs and on acid-treated SWCNTs were found undergoing apoptosis with damaged cell membrane and condensed chromatin. The result of an immunocytochemical test showed that both “dot-like” and “dash-like” focal adhesion kinases (FAKs) mainly distributed at the periphery of cells cultured on SWCNTs, while “dot-like” FAKs distributed in the whole cell body of cells cultured on MWCNTs. We therefore hypothesize that FAK expression might play a key role in controlling cell viability for cells cultured on CNT scaffolds. [Copyright &y& Elsevier] ) |
RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1016/j.carbon.2007.12.015
)
)
[Languages] => Array
(
[0] => Array
(
[Code] => eng
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 8
[StartPage] => 453
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => CARBON nanotubes
[Type] => general
)
[1] => Array
(
[SubjectFull] => VACUUM
[Type] => general
)
[2] => Array
(
[SubjectFull] => CERVICAL cancer
[Type] => general
)
[3] => Array
(
[SubjectFull] => FOCAL adhesion kinase
[Type] => general
)
[4] => Array
(
[SubjectFull] => CELL culture
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Influence of carbon nanotube scaffolds on human cervical carcinoma HeLa cell viability and focal adhesion kinase expression
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Zhang, Xiaoke
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Wang, Xuefeng
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lu, Qinghua
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Fu, Chuanlong
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 03
[Text] => Mar2008
[Type] => published
[Y] => 2008
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 00086223
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 46
)
[1] => Array
(
[Type] => issue
[Value] => 3
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Carbon
[Type] => main
)
)
)
)
)
)
)
|
IllustrationInfo |