دورية أكاديمية
In Vitro Migration of L3T4+ Cloned T cells to Epidermis: Possible Role for Keratinocyte-Derived Factors.
| العنوان: | In Vitro Migration of L3T4+ Cloned T cells to Epidermis: Possible Role for Keratinocyte-Derived Factors. |
|---|---|
| المؤلفون: | Shiohara, Tetsuo1, Moriya, Noriko1, Gotoh, Chie1, Gomi, Toshihiko1, Nigashima, Masaji1 |
| المصدر: | Journal of Investigative Dermatology. Mar1989, Vol. 92 Issue 3, p360-365. 6p. |
| مصطلحات موضوعية: | *ANTIGENS, *EPIDERMIS, *CELLS, *BIOLOGICAL assay, *LYMPH nodes, *KERATINOCYTES |
| مستخلص: | Three types of L3T4+ cloned T cells with different antigen specificities, auto-, allo-, and antigen-reactive, were characterized with respect to their migratory potential using an in vitro migration assay under agar gel. Autoreactive T cells, BB5, and alloreactive T cells, SK 1, both of which have been proved to be epidermotropic in vivo, showed specific directional migration to the epidermis, whereas no directional migration was seen with non-epidermotropic cloned T cells and freshly isolated lymph node T cells. Both BB5 and SK 1 cells were equally attracted to all the epidermal fragments tested regardless of their I-A antigens. The directional migration of BB5 cells to the epidermis was significantly inhibited by the co-cultivation with the epidermis, but not the dermis. Studies with cell lines, the conditioned media (CM), and recombinant interleukin (IL) 1, 2, and 3 revealed that BB5 cells were chemotactically attracted to a transformed keratinocyte cell line PAM212 and, to a lesser extent, to the CM from PAM212 and IL-2, but not to IL-1 and IL 3. These results suggest that epidermotropic T cells may be preferentially trapped in an area with a high concentration of keratinocyte-derived growth factors as well as IL-2. [ABSTRACT FROM AUTHOR] |
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