التفاصيل البيبلوغرافية
| العنوان: |
Construction of Peptide Library in Mammalian Cells by dsDNA-Based Strategy |
| المؤلفون: |
Weijun Su (3350900), Yi Wang (32470), Siqi Zou (14309646), Yanjie Zhao (591907), Yifan Li (327507), Chunze Zhang (4630786), Xiaojing Guo (494536), Shuai Li (65944) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Genetics, Molecular Biology, Physiology, Biotechnology, Immunology, Cancer, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, terminal degenerate codons, store coding information, screen functional peptides, hardly achieve function, great application potential, generate peptide libraries, build peptide libraries, different display technologies, work establishes dsdnas, innovative biological parts, gate genetic circuits, dbag peptide libraries, based peptide screening, dbag peptide library, peptide library, biological parts, peptide length, interacting peptide |
| الوصف: |
While different display technologies, represented by phage display, have been widely used in drug discovery, they still can hardly achieve function-based peptide screening, which in most cases is performed in mammalian cells. And most attempts to screen functional peptides with mammalian platforms utilized plasmids to store coding information. Our previous work established double-stranded DNAs (dsDNAs) as innovative biological parts to implement AND-gate genetic circuits in mammalian cells. In the current study, we employ dsDNAs with terminal NNK degenerate codons to implement AND-gate genetic circuits and generate peptide libraries in mammalian cells. This dsDNA-based AND-gate (DBAG) peptide library construction strategy is easy to perform, requiring only PCR reaction and cell transfection. High-throughput sequencing (HTS) and single-cell sequencing results revealed both peptide length and amino acid sequence diversity of DBAG peptide libraries. Moreover, as a feasibility test of this strategy, we identified an MDM2-interacting peptide by applying the DBAG peptide library to a mammalian cell-based two-hybrid system. Our work establishes dsDNAs with terminal degenerate codons as biological parts to build peptide libraries in mammalian cells, which may have great application potential in the future. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Construction_of_Peptide_Library_in_Mammalian_Cells_by_dsDNA-Based_Strategy/21791190Test |
| DOI: |
10.1021/acsomega.2c06402.s001 |
| الإتاحة: |
https://doi.org/10.1021/acsomega.2c06402.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.7FDACD63 |
| قاعدة البيانات: |
BASE |
