دورية أكاديمية
Methylome and transcriptome maps of human visceral and subcutaneous adipocytes reveal key epigenetic differences at developmental genes
| العنوان: | Methylome and transcriptome maps of human visceral and subcutaneous adipocytes reveal key epigenetic differences at developmental genes |
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| المؤلفون: | Bradford, ST, Nair, SS, Statham, AL, van Dijk, SJ, Peters, TJ, Anwar, F, French, HJ, von Martels, JZH, Sutcliffe, B, Maddugoda, MP, Peranec, M, Varinli, H, Arnoldy, R, Buckley, M, Ross, JP, Zotenko, E, Song, JZ, Stirzaker, C, Bauer, DC, Qu, W, Swarbrick, MM, Lutgers, HL, Lord, RV, Samaras, K, Molloy, PL, Clark, SJ |
| المصدر: | urn:ISSN:2045-2322 ; Scientific Reports, 9, 1, 9511 |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2019 |
| المجموعة: | UNSW Sydney (The University of New South Wales): UNSWorks |
| مصطلحات موضوعية: | Genetics, Obesity, Diabetes, Nutrition, 1 Underpinning research, 2.1 Biological and endogenous factors, 2 Aetiology, 1.1 Normal biological development and functioning, Metabolic and endocrine, Adipocytes, Adult, Binding Sites, Body Mass Index, DNA Methylation, Down-Regulation, Epigenesis, Genetic, Female, Gene Expression Regulation, Developmental, Humans, Intra-Abdominal Fat, Middle Aged, Regulatory Elements, Transcriptional, Subcutaneous Fat, Transcription Factors, Transcriptome, Up-Regulation |
| الوصف: | Adipocytes support key metabolic and endocrine functions of adipose tissue. Lipid is stored in two major classes of depots, namely visceral adipose (VA) and subcutaneous adipose (SA) depots. Increased visceral adiposity is associated with adverse health outcomes, whereas the impact of SA tissue is relatively metabolically benign. The precise molecular features associated with the functional differences between the adipose depots are still not well understood. Here, we characterised transcriptomes and methylomes of isolated adipocytes from matched SA and VA tissues of individuals with normal BMI to identify epigenetic differences and their contribution to cell type and depot-specific function. We found that DNA methylomes were notably distinct between different adipocyte depots and were associated with differential gene expression within pathways fundamental to adipocyte function. Most striking differential methylation was found at transcription factor and developmental genes. Our findings highlight the importance of developmental origins in the function of different fat depots. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/1959.4/unsworks_63991Test; https://unsworks.unsw.edu.au/bitstreams/c8d1a7c4-32d7-47f5-9d9c-9dde72ad3df6/downloadTest; https://doi.org/10.1038/s41598-019-45777-wTest |
| DOI: | 10.1038/s41598-019-45777-w |
| الإتاحة: | https://doi.org/10.1038/s41598-019-45777-wTest http://hdl.handle.net/1959.4/unsworks_63991Test https://unsworks.unsw.edu.au/bitstreams/c8d1a7c4-32d7-47f5-9d9c-9dde72ad3df6/downloadTest |
| حقوق: | open access ; https://purl.org/coar/access_right/c_abf2Test ; CC BY ; https://creativecommons.org/licenses/by/4.0Test/ ; free_to_read |
| رقم الانضمام: | edsbas.F2698502 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-019-45777-w |
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