دورية أكاديمية
Mouse Model of Nitrogen Mustard Ocular Surface Injury Characterization and Sphingolipid Signaling
| العنوان: | Mouse Model of Nitrogen Mustard Ocular Surface Injury Characterization and Sphingolipid Signaling |
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| المؤلفون: | Sandip K. Basu, Amanda Prislovsky, Nataliya Lenchik, Daniel J. Stephenson, Rajesh Agarwal, Charles E. Chalfant, Nawajes Mandal |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 2, p 742 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | nitrogen mustard (NM), NM eye injury, mouse model, vesicating injury, ocular surface injury, inflammation, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Vesicating chemicals like sulfur mustard (SM) or nitrogen mustard (NM) can cause devastating damage to the eyes, skin, and lungs. Eyes, being the most sensitive, have complicated pathologies that can manifest immediately after exposure (acute) and last for years (chronic). No FDA-approved drug is available to be used as medical counter measures (MCMs) against such injuries. Understanding the pathological mechanisms in acute and chronic response of the eye is essential for developing effective MCMs. Here, we report the clinical and histopathological characterization of a mouse model of NM-induced ocular surface injury (entire surface) developed by treating the eye with 2% ( w / v ) NM solution for 5 min. Unlike the existing models of specific injury, our model showed severe ocular inflammation, including the eyelids, structural deformity of the corneal epithelium and stroma, and diminished visual and retinal functions. We also observed alterations of the inflammatory markers and their expression at different phases of the injury, along with an activation of acidic sphingomyelinase (aSMase), causing an increase in bioactive sphingolipid ceramide and a reduction in sphingomyelin levels. This novel ocular surface mouse model recapitulated the injuries reported in human, rabbit, and murine SM or NM injury models. NM exposure of the entire ocular surface in mice, which is similar to accidental or deliberate exposure in humans, showed severe ocular inflammation and caused irreversible alterations to the corneal structure and significant vision loss. It also showed an intricate interplay between inflammatory markers over the injury period and alteration in sphingolipid homeostasis in the early acute phase. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/25/2/742Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/a16aa26df15e443489aea6c7bc121596Test |
| DOI: | 10.3390/ijms25020742 |
| الإتاحة: | https://doi.org/10.3390/ijms25020742Test https://doaj.org/article/a16aa26df15e443489aea6c7bc121596Test |
| رقم الانضمام: | edsbas.F3184413 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25020742 |