دورية أكاديمية
Photon-Weight-Reallocated Stimulated Emission Depletion Nanoscopy
| العنوان: | Photon-Weight-Reallocated Stimulated Emission Depletion Nanoscopy |
|---|---|
| المؤلفون: | Zhimin Zhang, Dazhao Zhu, Yubing Han, Minfei He, Liang Xu, Wenli Tao, Cuifang Kuang, Xu Liu |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Medicine, Microbiology, Cell Biology, Biotechnology, Cancer, Plant Biology, Space Science, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, stimulated emission depletion, preserving effective ones, honeycomb array detector, fluorescence lifetime detection, achieving maximum improvements, temporal photon weights, minimize background noise, photon weight reallocation, noise suppression, reallocation process, useless photons, lateral resolutions, individual photons, background ratio |
| الوصف: | A novel optimization concept called “photon weight reallocation” is proposed to evaluate the significance of individual photons in an image, filtering out useless photons while preserving effective ones. This process aims to minimize background noise and enhance the resolution of the image. By leveraging a honeycomb array detector and fluorescence lifetime detection, photon weight reallocation is implemented in stimulated emission depletion (STED) nanoscopy through the fusion of the spatial and temporal photon weights. This approach yields exceptional results in terms of background and noise suppression and resolution enhancement. The experimental results demonstrate significant improvements in both the signal-to-background ratio and peak signal-to-background ratio within dense sample areas of STED after applying the photon-weight-reallocation process, achieving maximum improvements by factors of 8.9 and 37.3, respectively. Besides, the axial and lateral resolutions of STED are improved by factors of 1.55 and 1.36, respectively, following this process. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Photon-Weight-Reallocated_Stimulated_Emission_Depletion_Nanoscopy/24539393Test |
| DOI: | 10.1021/acsphotonics.3c00922.s001 |
| الإتاحة: | https://doi.org/10.1021/acsphotonics.3c00922.s001Test https://figshare.com/articles/journal_contribution/Photon-Weight-Reallocated_Stimulated_Emission_Depletion_Nanoscopy/24539393Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.5C6275B7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsphotonics.3c00922.s001 |
|---|