دورية أكاديمية
The Added Value of a Collagenated Thermosensitive Bone Substitute as a Scaffold for Bone Regeneration
| العنوان: | The Added Value of a Collagenated Thermosensitive Bone Substitute as a Scaffold for Bone Regeneration |
|---|---|
| المؤلفون: | Charlotte Jeanneau, Jean-Hugues Catherine, Thomas Giraud, Romain Lan, Imad About |
| المصدر: | Materials, Vol 17, Iss 3, p 625 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Technology LCC:Electrical engineering. Electronics. Nuclear engineering LCC:Engineering (General). Civil engineering (General) LCC:Microscopy LCC:Descriptive and experimental mechanics |
| مصطلحات موضوعية: | thermosensitive collagenated bone substitute, bone grafting, stem cell recruitment, angiogenic, osteogenic potential, Technology, Electrical engineering. Electronics. Nuclear engineering, TK1-9971, Engineering (General). Civil engineering (General), TA1-2040, Microscopy, QH201-278.5, Descriptive and experimental mechanics, QC120-168.85 |
| الوصف: | A pre-hydrated thermosensitive collagenated biomaterial which sets at body temperature and maintains the space of the missing alveolar bone volume, OsteoBiol GTO® (GTO), has been released as a bone substitute. This study was designed to check its angiogenic and osteogenic potentials compared to OsteoBiol Gen-Os® (Gen-Os) and Geistlich Bio-Oss® (Bio-Oss). Samples of materials were incubated in culture media to obtain the extracts. Collagen release was measured in the extracts, which were used to investigate human periodontal ligament (hPDL) cell proliferation (MTT), colonization (Scratch assays) and growth factor release (ELISA). The effects on endothelial cell proliferation (MTT) and organization (Matrigel® assays) were also studied. Finally, endothelial and mesenchymal Stem Cell (hMSC) recruitment (Boyden Chambers) were investigated, and hMSC Alkaline Phosphatase (ALP) activity was measured. A higher collagen concentration was found in GTO extract, which led to significantly higher hPDL cell proliferation/colonization. All materials increased VEGF/FGF-2 growth factor secretion, endothelial cell recruitment, proliferation, and organization, but the increase was highest with GTO. All materials increased hMSC recruitment and ALP activity. However, the increase was highest with collagenated GTO and Gen-Os, which enhanced C5a and BMP-2 secretion. Overall, GTO has higher angiogenic/osteogenic potentials than the collagenated Gen-Os and the anorganic Bio-Oss. It provides a suitable scaffold for endothelial and mesenchymal stem cell recruitment, which represent essential bone regeneration requirements. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 17030625 1996-1944 |
| العلاقة: | https://www.mdpi.com/1996-1944/17/3/625Test; https://doaj.org/toc/1996-1944Test |
| DOI: | 10.3390/ma17030625 |
| الوصول الحر: | https://doaj.org/article/dac1abc286454cf2b2d8a859b679b290Test |
| رقم الانضمام: | edsdoj.1abc286454cf2b2d8a859b679b290 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17030625 19961944 |
|---|---|
| DOI: | 10.3390/ma17030625 |