Immunometabolic profiling of T cells from patients with relapsing-remitting multiple sclerosis reveals an impairment in glycolysis and mitochondrial respiration
| العنوان: | Immunometabolic profiling of T cells from patients with relapsing-remitting multiple sclerosis reveals an impairment in glycolysis and mitochondrial respiration |
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| المؤلفون: | Claudia La Rocca, Ilaria Cerillo, Giorgia Teresa Maniscalco, Giuseppe Orefice, Francesco Perna, Mario Galgani, Veronica De Rosa, Giuseppe Matarese, Ciro Florio, Fortunata Carbone, Antonio Uccelli, Alessandra Colamatteo, Andrea Visconti, Diego Centonze, Vincenzo Brescia Morra, Salvatore Longobardi, Marco Salvetti, Roberta Lanzillo |
| المساهمون: | La Rocca, Claudia, Carbone, Fortunata, De Rosa, Veronica, Colamatteo, Alessandra, Galgani, Mario, Perna, Francesco, Lanzillo, Roberta, Brescia Morra, Vincenzo, Orefice, Giuseppe, Cerillo, Ilaria, Florio, Ciro, Maniscalco, Giorgia Teresa, Salvetti, Marco, Centonze, Diego, Uccelli, Antonio, Longobardi, Salvatore, Visconti, Andrea, Matarese, Giuseppe |
| المصدر: | Metabolism, clinical and experimental (Online) 77 (2017): 39–46. doi:10.1016/j.metabol.2017.08.011 info:cnr-pdr/source/autori:La Rocca C.; Carbone F.; De Rosa V.; Colamatteo A.; Galgani M.; Perna F.; Lanzillo R.; Brescia Morra V.; Orefice G.; Cerillo I.; Florio C.; Maniscalco G.T.; Salvetti M.; Centonze D.; Uccelli A.; Longobardi S.; Visconti A.; Matarese G./titolo:Immunometabolic profiling of T cells from patients with relapsing-remitting multiple sclerosis reveals an impairment in glycolysis and mitochondrial respiration/doi:10.1016%2Fj.metabol.2017.08.011/rivista:Metabolism, clinical and experimental (Online)/anno:2017/pagina_da:39/pagina_a:46/intervallo_pagine:39–46/volume:77 Metabolism |
| بيانات النشر: | W.B. Saunders, [Philadelphia, Pa.] , Stati Uniti d'America, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, E2, exone2, Glycolysi, RANKL, receptor activator of nuclear factor kappaB ligand, BMI, body mass index, Endocrinology, Diabetes and Metabolism, T-Lymphocytes, MPO, myeloperoxidase, Adipocytokine, Mitochondrion, Relapsing-Remitting, RR, relapsing remitting, Lymphocyte Activation, Oxidative Phosphorylation, Immune tolerance, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, OCR, oxygen consumption rate, CIA, collagen-induced arthritis, Adipocytokines, Tconv, conventional T cells, EAE, experimental autoimmune encephalomyelitis, DLST, dihydrolipoamide succinyltransferase, Glucose Transporter Type 1, TCR, T cell receptor, sICAM-1, soluble ICAM-1, adipocytokines, ifn beta-1a, metabolism, multiple sclerosis, adult, case-control studies, female, glucose transporter type 1, humans, interferon beta-1a, lymphocyte activation, male, middle aged, mitochondria, multiple sclerosis, relapsing-remitting, oxidative phosphorylation, t-lymphocytes, young adult, glycolysis, endocrinology, diabetes and metabolism, endocrinology, MCP-1, monocyte chemoattractant protein-1, Middle Aged, sTNF-R, soluble tumor necrosis factor receptor, 3. Good health, Mitochondria, Diabetes and Metabolism, medicine.anatomical_structure, IFN beta-1a, Metabolism, Multiple sclerosis, sLeptinR, soluble leptin receptor, Female, Settore MED/26 - Neurologia, T1D, type 1 diabetes, Case-Control Studie, Glycolysis, Interferon beta-1a, Human, medicine.drug, Adult, medicine.medical_specialty, T cell, Oxidative phosphorylation, Biology, CNS, central nervous system, Article, MS, multiple sclerosis, 03 medical and health sciences, Young Adult, Multiple Sclerosis, Relapsing-Remitting, iTreg, inducible regulatory T cells, Immune system, DLAT, dihydrolipoamide S-acetyltransferase, Internal medicine, medicine, Humans, Multiple sclerosi, IFN, interferon, Th, T helper, PBMCs, peripheral blood mononuclear cells, Hexokinase, ECAR, extracellular acidification rate, Treg, regulatory T cells, Citric acid cycle, 030104 developmental biology, T-Lymphocyte, OPG, osteoprotegerin, chemistry, Case-Control Studies, sCD40L, soluble CD40 ligand, 030217 neurology & neurosurgery |
| الوصف: | Background Metabolic reprogramming is shaped to support specific cell functions since cellular metabolism controls the final outcome of immune response. Multiple sclerosis (MS) is an autoimmune disease resulting from loss of immune tolerance against central nervous system (CNS) myelin. Metabolic alterations of T cells occurring during MS are not yet well understood and their studies could have relevance in the comprehension of the pathogenetic events leading to loss of immune tolerance to self and to develop novel therapeutic strategies aimed at limiting MS progression. Methods and Results In this report, we observed that extracellular acidification rate (ECAR) and oxygen consumption rate (OCR), indicators of glycolysis and oxidative phosphorylation, respectively, were impaired during T cell activation in naive-to-treatment relapsing remitting (RR)MS patients when compared with healthy controls. These results were also corroborated at biochemical level by a reduced expression of the glycolitic enzymes aldolase, enolase 1, hexokinase I, and by reduction of Krebs cycle enzymes dihydrolipoamide- S -acetyl transferase (DLAT) and dihydrolipoamide-S-succinyl transferase (DLST). Treatment of RRMS patients with interferon beta-1a (IFN beta-1a) was able to restore T cell glycolysis and mitochondrial respiration as well as the amount of the metabolic enzymes to a level comparable to that of healthy controls. These changes associated with an up-regulation of the glucose transporter-1 (GLUT-1), a key element in intracellular transport of glucose. Conclusions Our data suggest that T cells from RRMS patients display a reduced engagement of glycolysis and mitochondrial respiration, reversible upon IFN beta-1a treatment, thus suggesting an involvement of an altered metabolism in the pathogenesis of MS. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5234b64cc5175a03d2ed3e047ea2212cTest https://publications.cnr.it/doc/383885Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5234b64cc5175a03d2ed3e047ea2212c |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |