دورية أكاديمية
Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis
| العنوان: | Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis |
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| المؤلفون: | Qizhao Tan, Feng Li, Ke Zhang, Zhongjun Liu, Yun Tian, Tengjiao Zhu |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Cell Biology, Molecular Biology, Physiology, Ecology, Science Policy, Biological Sciences not elsewhere classified, validation experiments suggested, promising therapeutic target, prevalent debilitating whole, evaluate molecular biomarkers, rats using immunohistochemistry, complement pathways plays, oa rats compared, control rats, oa compared, subchondral bone, significantly increased, several tissues, results indicated, proteomics analysis, proteomic studies, proteomic analysis, protein profiles, protein involved, osteoarthritis osteoarthritis, joint disorder, induced oa, growing number, control group |
| الوصف: | Osteoarthritis (OA) is a prevalent debilitating whole-joint disorder. Currently, a growing number of proteomic studies have been performed to evaluate molecular biomarkers in several tissues from OA patients; however, little is known about the protein profiles in subchondral bone of OA. In this study, proteomic analysis was performed on subchondral bone from patients with OA to identify differentially expressed proteins (DEPs). Bioinformatics tools were used to further investigate these DEPs. Thereafter, DEPs were validated in the samples from patients with OA, as well as in bilateral ovariectomy-induced OA (OVX-OA) rats using immunohistochemistry. A comprehensive subchondral bone proteome profile of patients with OA was constructed. Additionally, biological information analysis showed that a majority of DEPs participated in the dysregulation of the complement and coagulation cascades. The validation experiments suggested that SerpinA5, the protein involved in the complement and coagulation cascades, was significantly increased in severely damaged subchondral bone of patients with OA compared to the control group. Furthermore, the increase of SerpinA5 in OVX-OA rats compared to control rats was also confirmed. Our results indicated that the dysregulation of coagulation and complement pathways plays a role in the progression of OA, and it provides a promising therapeutic target of OA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Proteomics_Analysis_of_Knee_Subchondral_Bone_Identifies_Differentially_Expressed_Proteins_Associated_with_Osteoarthritis/24982816Test |
| DOI: | 10.1021/acs.jproteome.3c00584.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jproteome.3c00584.s001Test https://figshare.com/articles/journal_contribution/Proteomics_Analysis_of_Knee_Subchondral_Bone_Identifies_Differentially_Expressed_Proteins_Associated_with_Osteoarthritis/24982816Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.3F9792BA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jproteome.3c00584.s001 |
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