دورية أكاديمية
Effect of nitrogen fixation enhancing type SEN1 gene on soybean growth
العنوان: | Effect of nitrogen fixation enhancing type SEN1 gene on soybean growth |
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المؤلفون: | Aya Shimomura, Yuki Nishida, Shion Yamamoto, Norio Suganuma, Satoshi Watanabe, Toyoaki Anai, Susumu Arima, Akiyoshi Tominaga, Akihiro Suzuki |
المصدر: | Plant Production Science, Vol 27, Iss 2, Pp 137-149 (2024) |
بيانات النشر: | Taylor & Francis Group, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Plant culture |
مصطلحات موضوعية: | Glycine max, symbiosis, nitrogen fixation, SEN1, near-isogenic lines (NILs), plant growth, vacuolar iron transporter-like protein (VTL), Plant culture, SB1-1110 |
الوصف: | ABSTRACTIt is well known that the growth of legumes is greatly affected not only by fertilizer nitrogen but also by fixed nitrogen by symbiosis with rhizobia. We have reported that a polymorphism in SEN1, which is essential for nitrogen fixation in Lotus japonicus, could be used to enhance nitrogen fixation and plant growth. In this study, we examined whether the same strategy could be applied to Glycine max. Sequencing of orthologs of the LjSEN1 gene in 38 soybean cultivars revealed that the polymorphism occurred at Glyma.08g076300. We defined the standard sequence that most of the cultivars retained as Peking type SEN1, and the mutated sequence found in Enrei as Enrei type SEN1. To investigate the effect of genotype differences, we generated near-isogenic lines by crossing Enrei with Fukuyutaka, which has Peking type SEN1, and analyzed their characteristics. The results showed that Enrei type enhanced nitrogen fixation activity and promoted plant growth. Since soybean SEN1 has been reported to be a homolog of vacuolar iron transporter-like protein, we investigated the iron content in the nodules. In the result, the iron content in the nodules of Enrei type was significantly higher than that of Peking type. Iron is known to be present in the active center of nitrogenase, and the high iron content may have contributed to the increased nitrogen fixation activity. These results suggest that Enrei type SEN1 is a nitrogen fixation enhancing gene and that introduction of Enrei type SEN1 may reduce nitrogen fertilizer application and increase yield. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1343943X 1349-1008 1343-943X |
العلاقة: | https://doaj.org/toc/1343-943XTest; https://doaj.org/toc/1349-1008Test |
DOI: | 10.1080/1343943X.2024.2326643 |
الوصول الحر: | https://doaj.org/article/018adf7dbec34853819ebcc6feaec067Test |
رقم الانضمام: | edsdoj.018adf7dbec34853819ebcc6feaec067 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 1343943X 13491008 |
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DOI: | 10.1080/1343943X.2024.2326643 |