دورية أكاديمية
Bioorthogonal Reaction-Mediated Enzymatic Elongation-Driven Dendritic Nanoassembly for Genome-Wide Analysis of 5‑Hydroxymethyluracil in Breast Tissues
| العنوان: | Bioorthogonal Reaction-Mediated Enzymatic Elongation-Driven Dendritic Nanoassembly for Genome-Wide Analysis of 5‑Hydroxymethyluracil in Breast Tissues |
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| المؤلفون: | Su Jiang, Yanbo Cai, Qian-yi Zhang, Qian Liu, Zi-yue Wang, Chun-yang Zhang |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Medicine, Cell Biology, Genetics, Molecular Biology, Cancer, Plant Biology, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, related biological research, mediated hyperbranched amplification, mediated enzymatic elongation, holding great promise, driven dendritic nanoassembly, accurately detect even, breast cell lines, breast cancer patients, human breast tissues, breast tissues 5, 5 ‑ hydroxymethyluracil, cell level, cancer biomarker, wide analysis, various organisms, unsatisfactory sensitivity, ultralow background, oxidation derivative, normal persons, may serve, low specificity |
| الوصف: | 5-Hydroxymethyluracil (5hmU) is an oxidation derivative of thymine in the genomes of various organisms and may serve as both an epigenetic mark and a cancer biomarker. However, the current 5hmU assays usually have drawbacks of laborious procedures, low specificity, and unsatisfactory sensitivity. Herein, we demonstrate the click chemistry-mediated hyperbranched amplification-driven dendritic nanoassembly for genome-wide analysis of 5hmU in breast cell lines and human breast tissues. The proposed strategy possesses good selectivity, ultralow background, and high sensitivity with a detection limit of 83.28 aM. This method can accurately detect even a 0.001% 5hmU level in the mixture. Moreover, it can determine 5hmU at single-cell level and distinguish the expressions of 5hmU in tissues of normal persons and breast cancer patients, holding great promise in 5hmU-related biological research and clinical diagnosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Bioorthogonal_Reaction-Mediated_Enzymatic_Elongation-Driven_Dendritic_Nanoassembly_for_Genome-Wide_Analysis_of_5_Hydroxymethyluracil_in_Breast_Tissues/24512889Test |
| DOI: | 10.1021/acs.nanolett.3c03754.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.nanolett.3c03754.s001Test https://figshare.com/articles/journal_contribution/Bioorthogonal_Reaction-Mediated_Enzymatic_Elongation-Driven_Dendritic_Nanoassembly_for_Genome-Wide_Analysis_of_5_Hydroxymethyluracil_in_Breast_Tissues/24512889Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.4389668 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.nanolett.3c03754.s001 |
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