Evaluation of Pyrophosphate‐Driven Proton Pumps in Saccharomyces cerevisiae under Stress Conditions
| العنوان: | Evaluation of Pyrophosphate‐Driven Proton Pumps in Saccharomyces cerevisiae under Stress Conditions |
|---|---|
| المؤلفون: | Sreenivas, Krishnan, Eisentraut, Leon, Brink, Daniel P., Persson, Viktor C., Carlquist, Magnus, Gorwa-Grauslund, Marie F., van Niel, Ed W. J. |
| المصدر: | Microorganisms. 12(3) |
| مصطلحات موضوعية: | Saccharomyces cerevisiae, pH homeostasis, ATP, proton-translocating ATPase, proton-translocating pyrophosphatase, pHluorin, mQueen-2m, acetic acid, xylose, Naturvetenskap, Biologi, Mikrobiologi, Natural Sciences, Biological Sciences, Microbiology, Teknik, Industriell bioteknik, Engineering and Technology, Industrial Biotechnology |
| الوصف: | In Saccharomyces cerevisiae, pH homeostasis is reliant on ATP due to the use of proton-translocating ATPase (H+-ATPase) which constitutes a major drain within cellular ATP supply. Here, an exogenous proton-translocating pyrophosphatase (H+-PPase) from Arabidopsis thaliana, which uses inorganic pyrophosphate (PPi) rather than ATP, was evaluated for its effect on reducing the ATP burden. The H+-Ppase was localized to the vacuolar membrane or to the cell membrane, and their impact was studied under acetate stress at a low pH. Biosensors (pHluorin and mQueen-2m) were used to observe changes in intracellular pH (pHi) and ATP levels during growth on either glucose or xylose. A significant improvement of 35% in the growth rate at a pH of 3.7 and 6 g·L−1 acetic acid stress was observed in the vacuolar membrane H+-PPase strain compared to the parent strain. ATP levels were elevated in the same strain during anaerobic glucose and xylose fermentations. During anaerobic xylose fermentations, co-expression of pHluorin and a vacuolar membrane H+-PPase improved the growth characteristics by means of an improved growth rate (11.4%) and elongated logarithmic growth duration. Our study identified a potential method for improving productivity in the use of S. cerevisiae as a cell factory under the harsh conditions present in industry. |
| وصف الملف: | electronic |
| الوصول الحر: | https://lup.lub.lu.se/record/94d47e76-daa4-463f-8b8f-586d56878077Test https://portal.research.lu.se/files/177716503/Sreenivas_et_al_2024_microorganisms-12-00625.pdfTest http://dx.doi.org/10.3390/microorganisms12030625Test |
| قاعدة البيانات: | SwePub |
| تدمد: | 20762607 |
|---|---|
| DOI: | 10.3390/microorganisms12030625 |