دورية أكاديمية

A Novel Alphabaculovirus from the Soybean Looper, Chrysodeixis includens, that Produces Tetrahedral Occlusion Bodies and Encodes Two Copies of he65

التفاصيل البيبلوغرافية
العنوان: A Novel Alphabaculovirus from the Soybean Looper, Chrysodeixis includens, that Produces Tetrahedral Occlusion Bodies and Encodes Two Copies of he65
المؤلفون: Robert L. Harrison, Daniel L. Rowley, Holly J. R. Popham
المصدر: Viruses; Volume 11; Issue 7; Pages: 579
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2019
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: soybean looper, Chrysodeixis includens, baculovirus, occlusion body, polyhedrin, lef-12, he65, DNA ligase 3
جغرافية الموضوع: agris
الوصف: Isolates of the alphabaculovirus species, Chrysodeixis includens nucleopolyhedrovirus, have been identified that produce polyhedral occlusion bodies and infect larvae of the soybean looper, Chrysodeixis includens. In this study, we report the discovery and characterization of a novel C. includens-infecting alphabaculovirus, Chrysodeixis includens nucleopolyhedrovirus #1 (ChinNPV#1), that produces tetrahedral occlusion bodies. In bioassays against C. includens larvae, ChinNPV #1 exhibited a degree of pathogenicity that was similar to that of other ChinNPV isolates, but killed larvae more slowly. The host range of ChinNPV#1 was found to be very narrow, with no indication of infection occurring in larvae of Trichoplusia ni and six other noctuid species. The ChinNPV#1 genome sequence was determined to be 130,540 bp, with 126 open reading frames (ORFs) annotated but containing no homologous repeat (hr) regions. Phylogenetic analysis placed ChinNPV#1 in a clade with other Group II alphabaculoviruses from hosts of lepidopteran subfamily Plusiinae, including Chrysodeixis chalcites nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus. A unique feature of the ChinNPV#1 genome was the presence of two full-length copies of the he65 ORF. The results indicate that ChinNPV#1 is related to, but distinct from, other ChinNPV isolates.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
العلاقة: Insect Viruses; https://dx.doi.org/10.3390/v11070579Test
DOI: 10.3390/v11070579
الإتاحة: https://doi.org/10.3390/v11070579Test
حقوق: https://creativecommons.org/licenses/by/4.0Test/
رقم الانضمام: edsbas.558E6206
قاعدة البيانات: BASE