التفاصيل البيبلوغرافية
| العنوان: |
Automated Spatially Targeted Optical Microproteomics Investigates Inflammatory Lesions In Situ |
| المؤلفون: |
Bocheng Yin (1481101), Laura R. Caggiano (11338366), Rung-Chi Li (11338369), Emily McGowan (11338372), Jeffrey W. Holmes (6087089), Sarah E. Ewald (8260788) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Physiology, Biotechnology, Immunology, Developmental Biology, Inorganic Chemistry, Chemical Sciences not elsewhere classified, report custom codes, Automated Spatially Targeted Optica., region, tissue, Situ Tissue microenvironment properties, LC, target cell types, label-free quantification methodology, protein expression |
| الوصف: |
Tissue microenvironment properties like blood flow, extracellular matrix, or proximity to immune-infiltrate are important regulators of cell biology. However, methods to study regional protein expression in the native tissue environment are limited. To address this need, we developed a novel approach to visualize, purify, and measure proteins in situ using automated spatially targeted optical microproteomics (AutoSTOMP). Here, we report custom codes to specify regions of heterogeneity in a tissue section and UV-biotinylate proteins within those regions. We have developed liquid chromatography–mass spectrometry (LC–MS)/MS-compatible biochemistry to purify those proteins and label-free quantification methodology to determine protein enrichment in target cell types or structures relative to nontarget regions in the same sample. These tools were applied to (a) identify inflammatory proteins expressed by CD68 + macrophages in rat cardiac infarcts and (b) characterize inflammatory proteins enriched in IgG4 + lesions in human esophageal tissues. These data indicate that AutoSTOMP is a flexible approach to determine regional protein expression in situ on a range of primary tissues and clinical biopsies where current tools and sample availability are limited. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Automated_Spatially_Targeted_Optical_Microproteomics_Investigates_Inflammatory_Lesions_i_In_Situ_i_/16455571Test |
| DOI: |
10.1021/acs.jproteome.1c00505.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.jproteome.1c00505.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.95E2EE92 |
| قاعدة البيانات: |
BASE |
