دورية أكاديمية
Investigation of the effects of pinacidil and glibenclamide administration on HCN1, KCNT1, Kir 6.1, SUR1 gene expressions in hippocampus and cortex regions in epileptic rats
| العنوان: | Investigation of the effects of pinacidil and glibenclamide administration on HCN1, KCNT1, Kir 6.1, SUR1 gene expressions in hippocampus and cortex regions in epileptic rats |
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| المؤلفون: | Kılıç, Ümit, Soytürk, H. |
| بيانات النشر: | Ondokuz Mayis Universitesi |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | epilepsy, gene expressions, HCN1, KCNT1, Kir 6.1, SUR1, glibenclamide, hyperpolarization activated cyclic nucleotide gated channel, hyperpolarization activated cyclic nucleotide gated channel 1, inwardly rectifying potassium channel subunit Kir6.1, kcnt1 protein, penicillin derivative, pinacidil, sodium activated potassium channel, sulfonylurea receptor 1, unclassified drug, animal experiment, animal model, animal tissue, Article, brain cortex, comparative study, controlled study, drug mechanism, epileptic focus, gene expression level, hippocampus, male, nonhuman, rat |
| الوصف: | The purpose of this study was to look into the effects of pinacidil and glibenclamide on HCN1, KCNT1, Kir 6.1, and SUR1 gene expression in epileptic rats hippocampus and cortex. Male Wistar-Albino rats were used in this study. The drugs pinacidil and glibenclamide were utilized. Control, Epilepsy, Epilepsy-O, and Epilepsy-B were the five groups formed. The epileptic focus was created by intracortical administration of penicillin at a dose of 500 IU/2 ?l. Hippocampus and Cortex are removed from all animals and Kir 6.1, SUR1, HCN1, and KCNT1 gene expression levels were determined by qPCR. The SPSS 21 program was used for statistics. HCN1 gene expression level is equal in the hippocampus and cortex (p<0.05). KCNT1, SUR1, and KIR6.1 are more expressed in the hippocampus than in the cortex (p<0.05). In epilepsy groups, HCN1 gene expression level was found to be higher than KCNT1, SUR1, and KIR6.1 gene expression levels (p<0.05). KIR6.1, SUR1, gene expression levels decreased with the application of pinacidil and glibenclamide (p<0.05). SUR1 and KIR6.1 gene expression levels were considerably lower in the epilepsy pinacidil group compared to the other groups. The gene expression levels in the hippocampus were found to be considerably higher than in the cortex group, according to this study. The fact that HCN1 gene expression levels are significantly greater in both the brain and the hippocampus 24 hours following the commencement of epileptic convulsions suggests that preventive medication may be possible. © 2022 Ondokuz Mayis Universitesi. All rights reserved. ; 2019.10.01.1408 ; This study was financed by the Abant İzzet Baysal University Research Foundation (BAP-grant number: 2019.10.01.1408). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1309-4483 |
| العلاقة: | Journal of Experimental and Clinical Medicine (Turkey); Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.52142/omujecm.39.3.52Test; https://hdl.handle.net/20.500.12684/13611Test; 39; 868; 873; 2-s2.0-85142021264 |
| DOI: | 10.52142/omujecm.39.3.52 |
| الإتاحة: | https://doi.org/20.500.12684/13611Test https://doi.org/10.52142/omujecm.39.3.52Test https://hdl.handle.net/20.500.12684/13611Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F1726EF0 |
| قاعدة البيانات: | BASE |
| تدمد: | 13094483 |
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| DOI: | 10.52142/omujecm.39.3.52 |