دورية أكاديمية
Interbacterial Biofilm Competition through a Suite of Secreted Metabolites
| العنوان: | Interbacterial Biofilm Competition through a Suite of Secreted Metabolites |
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| المؤلفون: | Soniya Rayi, Yanyao Cai, Jennifer L. Greenwich, Clay Fuqua, Joseph P. Gerdt |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Pharmacology, Ecology, Marine Biology, Infectious Diseases, Virology, Astronomical and Space Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, genetic knockout experiments, interbacterial biofilm competition, species biofilm regulation, inhibiting biofilm formation, siderophores still secreted, pseudomonas aeruginosa inhibits, species biofilm, biofilm formation, tumefaciens <, n <, fohorn ), dihydroaeruginoic acid, compensatory overproduction, chemical nature, chemical foundation, antibiofilm metabolite, antagonistic interactions, agrobacterium tumefaciens, >< sup |
| الوصف: | Polymicrobial biofilms are ubiquitous, and the complex interspecies interactions within them are cryptic. We discovered the chemical foundation of antagonistic interactions in a model dual-species biofilm in which Pseudomonas aeruginosa inhibits the biofilm formation of Agrobacterium tumefaciens. Three known siderophores produced by P. aeruginosa (pyoverdine, pyochelin, and dihydroaeruginoic acid) were each capable of inhibiting biofilm formation. Surprisingly, a mutant that was incapable of producing these siderophores still secreted an antibiofilm metabolite. We discovered that this inhibitor was N 5 -formyl- N 5 -hydroxy-l-ornithine (fOHOrn)a precursor in pyoverdine biosynthesis. Unlike the siderophores, this inhibitor did not appear to function via extracellular metal sequestration. In addition to this discovery, the compensatory overproduction of a new biofilm inhibitor illustrates the risk of pleiotropy in genetic knockout experiments. In total, this work lends new insight into the chemical nature of dual-species biofilm regulation and reveals a new naturally produced inhibitor of A. tumefaciens biofilm formation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Interbacterial_Biofilm_Competition_through_a_Suite_of_Secreted_Metabolites/25049789Test |
| DOI: | 10.1021/acschembio.3c00655.s001 |
| الإتاحة: | https://doi.org/10.1021/acschembio.3c00655.s001Test https://figshare.com/articles/journal_contribution/Interbacterial_Biofilm_Competition_through_a_Suite_of_Secreted_Metabolites/25049789Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.E63F4323 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acschembio.3c00655.s001 |
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