رسالة جامعية
The study of protein interaction between harpinPss and HARP by means of truncated HRAP
| العنوان: | The study of protein interaction between harpinPss and HARP by means of truncated HRAP |
|---|---|
| المؤلفون: | Chou, Hung-wen |
| مرشدي الرسالة: | Ching-mei Hsu, Mang-jye Ger¡@, Zin-huang Liu |
| بيانات النشر: | NSYSU, 2006. |
| سنة النشر: | 2006 |
| المجموعة: | NSYSU Electronic Thesis and Dissertation Archive |
| Original Material: | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0710106-164521Test |
| مصطلحات موضوعية: | hypersensitive response, harpinPss, hypersensitive response assisting protein (HRAP), protein truncation |
| الوصف: | HarpinPss, a proteinaceous elicitor from Pseudomonas syringae pv. syringae, is a glycine-rich, cysteine-lacking, heat-stable protein. It can elicit the hypersensitive response (HR) when delivered to the surface of plant cells. HRAP (hypersensitive response assisting protein) is an amphipathic protein purified from sweet pepper and could intensify harpinPss¡Vmediated HR in sweet pepper. In the previous research, harpinPss was present as monomer, dimer, trimer, tetramer, and ocatamer forms in neutral pH buffer. Only monomer and dimer forms of harpinPss induced hypersensitive response in nonhost plants. HRAP could cause multimeric forms of harpinPss dissociation into monomer forms. The interaction between HRAP and harpinPss is an important issue. HRAP contained three positively charged regions, a typical signal peptide and a cAMP-dependent phosphorylation site. In this study, these regions of HRAP would be truncated and identified whether these truncated HRAP fragments could promote harpinPss dissociation. Different combinations of truncated HRAP and harpinPss were used to identify the protein-interaction regions between two proteins. HarpinPss triggers HR via interaction with cAMP phosphorated region of HRAP and MAPK pathway transduction. When cAMP region of HRAP was truncated, harpinPss still triggers HR via polymerization and anchor on lipid bilayers to form an ion-conducting pore. |
| Original Identifier: | oai:NSYSU:etd-0710106-164521 |
| نوع الوثيقة: | Text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الإتاحة: | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0710106-164521Test |
| حقوق: | unrestricted Copyright information available at source archive |
| رقم الانضمام: | edsndl.NSYSU.oai.NSYSU.etd.0710106.164521 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |