التفاصيل البيبلوغرافية
| العنوان: |
Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| المؤلفون: |
Anna von Schoenfeld, Peter Bronsert, Michael Poc, Andrew Fuller, Andrew Filby, Stefan Kraft, Konrad Kurowski, Kristin Sörensen, Julia Huber, Jens Pfeiffer, Michele Proietti, Verena Stehl, Martin Werner, Maximilian Seidl |
| المصدر: |
International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 223 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
immunofluorescence, immunohistochemistry, CyTOF, multiple staining, re-fixation, formalin, retrieval, crossreactivity, hydrophobic masking |
| جغرافية الموضوع: |
agris |
| الوصف: |
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Molecular Pathology, Diagnostics, and Therapeutics; https://dx.doi.org/10.3390/ijms23010223Test |
| DOI: |
10.3390/ijms23010223 |
| الإتاحة: |
https://doi.org/10.3390/ijms23010223Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.37BB8D8A |
| قاعدة البيانات: |
BASE |
