hCINAP serves a critical role in hypoxia-induced cardiomyocyte apoptosis via modulating lactate production and mitochondrial-mediated apoptosis signaling
العنوان: | hCINAP serves a critical role in hypoxia-induced cardiomyocyte apoptosis via modulating lactate production and mitochondrial-mediated apoptosis signaling |
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المؤلفون: | Yuqi Gao, Gang Xu, Zhibin Yuan, Xie Hebing |
المصدر: | Molecular Medicine Reports |
بيانات النشر: | D.A. Spandidos, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | Cancer Research, Lactate dehydrogenase A, Cell, lactate dehydrogenase A, Biochemistry, Mitochondria, Heart, Cell Line, Genetics, medicine, Humans, Myocytes, Cardiac, Viability assay, Lactic Acid, education, Molecular Biology, Caspase, Gene knockdown, education.field_of_study, human coilin interacting nuclear ATPase protein, lactate, biology, Chemistry, hypoxia, apoptosis, Articles, Cell cycle, Cell Hypoxia, Cell biology, Blot, DNA-Binding Proteins, medicine.anatomical_structure, Oncology, Apoptosis, biology.protein, Molecular Medicine, Signal Transduction |
الوصف: | Acute myocardial infarction (AMI) is a major cause of heart failure and is associated with insufficient myocardial oxygen supply. However, the molecular mechanisms underlying hypoxia‑induced cardiomyocyte apoptosis are not completely understood. In the present study, the role of human coilin interacting nuclear ATPase protein (hCINAP) in cardiomyocytes was investigated. AC16 cells were divided into the following four groups: i) Small interfering (si)RNA‑control (Ctrl); (ii) siRNA‑hCINAP; (iii) empty vector; and (iv) hCINAP‑Flag. Protein expression was assessed using western blotting. MTT and apoptosis assays were conducted to detect cell viability and apoptosis, respectively. CCK8 assays and apoptosis assays were used to detect cell viability and apoptosis, respectively. hCINAP promoter activity was examined by luciferase reporter assay. hCINAP expression was induced in a hypoxia‑inducible factor‑1α‑dependent manner under hypoxic conditions. Compared with the siRNA‑Ctrl group, hCINAP knockdown inhibited apoptosis, whereas compared with the vector group, hCINAP overexpression increased apoptosis under hypoxic conditions. Mechanistically, compared with the siRNA‑Ctrl group, hCINAP knockdown decreased hypoxia‑induced lactate accumulation via regulating lactate dehydrogenase A activity. Moreover, the results indicated that hCINAP was associated with mitochondrial‑mediated apoptosis via Caspase signaling. Collectively, the present study suggested that hCINAP was an important regulator in hypoxia‑induced apoptosis and may serve as a promising therapeutic target for AMI. |
اللغة: | English |
تدمد: | 1791-3004 1791-2997 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4acd011a748bc4b9d12ecb5dd533fb5Test http://europepmc.org/articles/PMC7723068Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....a4acd011a748bc4b9d12ecb5dd533fb5 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 17913004 17912997 |
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