دورية أكاديمية
Photon-Efficient Aberration Correction for 3D-STED Imaging of Thick Biological Specimens Using Sensorless Adaptive Optics
| العنوان: | Photon-Efficient Aberration Correction for 3D-STED Imaging of Thick Biological Specimens Using Sensorless Adaptive Optics |
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| المؤلفون: | Siqing Dai, Andrei Kobitski, Amirhossein Barati Sedeh, Süheyla Eroğlu-Kayıkçı, Lennart Hilbert, G. Ulrich Nienhaus |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Cell Biology, Biotechnology, Inorganic Chemistry, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, three spatial dimensions, spatial light modulators, intricate phase patterns, gold beads immobilized, focal intensity distribution, central intensity minimum, bottom surfaces enclosing, excellent imaging performance, efficient aberration correction, resolution imaging technique, larger sample depths, resolution enhancement, reflection imaging, sted imaging, zebrafish embryos, wavefront aberrations, thick specimens, successfully compensated, subcellular structure, powerful super, linear interpolation |
| الوصف: | Stimulated emission depletion (STED) microscopy is a powerful super-resolution imaging technique for investigating the subcellular structure of biological samples in all three spatial dimensions. Its application to thick specimens is challenging, however, as sample-induced optical aberrations distort the intricate phase patterns of the STED beams and, consequently, their focal intensity distribution. Notably, the central intensity minimum, which is essential for resolution enhancement, is lifted at larger sample depths. Here we present a technique for correction of STED beam aberrations featuring minimal light exposure of the samples to avoid photobleaching and phototoxicity. We quantify STED beam aberrations by using modal wavefront sensing with spatial light modulators (SLMs) as adaptive optics (AO) elements based on reflection imaging of gold beads immobilized at the top and bottom surfaces enclosing the thick (≥100 μm) biological specimen. Based on this information, wavefront aberrations can be successfully compensated for all depths by linear interpolation of the correction parameters. We demonstrate the excellent imaging performance of AO 3D stimulated emission double depletion (STEDD) microscopy on cell nuclei of zebrafish embryos. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Photon-Efficient_Aberration_Correction_for_3D-STED_Imaging_of_Thick_Biological_Specimens_Using_Sensorless_Adaptive_Optics/24954104Test |
| DOI: | 10.1021/acsphotonics.3c01558.s001 |
| الإتاحة: | https://doi.org/10.1021/acsphotonics.3c01558.s001Test https://figshare.com/articles/journal_contribution/Photon-Efficient_Aberration_Correction_for_3D-STED_Imaging_of_Thick_Biological_Specimens_Using_Sensorless_Adaptive_Optics/24954104Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.1AA86235 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsphotonics.3c01558.s001 |
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