التفاصيل البيبلوغرافية
| العنوان: |
Differentiation of Bacillus cereus and Bacillus thuringiensis Using Genome-Guided MALDI-TOF MS Based on Variations in Ribosomal Proteins |
| المؤلفون: |
Minling Chen, Xianhu Wei, Junhui Zhang, Huan Zhou, Nuo Chen, Juan Wang, Ying Feng, Shubo Yu, Jumei Zhang, Shi Wu, Qinghua Ye, Rui Pang, Yu Ding, Qingping Wu |
| المصدر: |
Microorganisms; Volume 10; Issue 5; Pages: 918 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
Bacillus cereus, Bacillus thuringiensis, genome-guided MALDI-TOF MS, ribosomal protein, high-throughput differentiation |
| جغرافية الموضوع: |
agris |
| الوصف: |
Bacillus cereus and B. thuringiensis are closely related species that are relevant to foodborne diseases and biopesticides, respectively. Unambiguous differentiation of these two species is crucial for bacterial taxonomy. As genome analysis offers an objective but time-consuming classification of B. cereus and B. thuringiensis, in the present study, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to accelerate this process. By combining in silico genome analysis and MALDI-TOF MS measurements, four species-specific peaks of B. cereus and B. thuringiensis were screened and identified. The species-specific peaks of B. cereus were m/z 3211, 6427, 9188, and 9214, and the species-specific peaks of B. thuringiensis were m/z 3218, 6441, 9160, and 9229. All the above peaks represent ribosomal proteins, which are conserved and consistent with the phylogenetic relationship between B. cereus and B. thuringiensis. The specificity of the peaks was robustly verified using common foodborne pathogens. Thus, we concluded that genome-guided MALDI-TOF MS allows high-throughput differentiation of B. cereus and B. thuringiensis and provides a framework for differentiating other closely related species. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
https://dx.doi.org/10.3390/microorganisms10050918Test |
| DOI: |
10.3390/microorganisms10050918 |
| الإتاحة: |
https://doi.org/10.3390/microorganisms10050918Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.B4C5B1D |
| قاعدة البيانات: |
BASE |
