دورية أكاديمية
Pharmacokinetics of Hydroxytyrosol and Its Sulfate and Glucuronide Metabolites after the Oral Administration of Table Olives to Sprague-Dawley Rats
| العنوان: | Pharmacokinetics of Hydroxytyrosol and Its Sulfate and Glucuronide Metabolites after the Oral Administration of Table Olives to Sprague-Dawley Rats |
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| المؤلفون: | Ivana Kundisová, Helena Colom, M. Emília Juan, Joana M. Planas |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Genetics, Pharmacology, Biotechnology, Environmental Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, tandem mass spectrometry, predominant metabolic pathway, mean residence time, despite extensive metabolism, liquid extraction followed, around 4 h, foods containing hydroxytyrosol, table olives provide, sustained plasma profile, noncompartmental pk analysis, two glucuronide conjugates, 85 mg hydroxytyrosol, table olives, liquid chromatography, 8 h, hydroxytyrosol remained, two sulfate, protecting activities, plasma samples, oral administration, may enhance, indicating sulfation, dawley rats |
| الوصف: | The pharmacokinetics (PK) of hydroxytyrosol and its metabolites were characterized following oral administration to Sprague-Dawley rats of 3.85 and 7.70 g of destoned Arbequina table olives/kg. Plasma samples were analyzed using a fully validated method consisting of liquid extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Noncompartmental PK analysis of hydroxytyrosol demonstrated linear PK between doses of 2.95 and 5.85 mg hydroxytyrosol/kg. Half-life was approximately 2.5 h, while mean residence time was around 4 h. Clearance occurred by conversion to two sulfate and two glucuronide conjugates. The area under the plasma concentration–time curve (AUC) ratios of metabolites versus parent hydroxytyrosol was approximately 7–9-fold for the sulfate and below 0.25 for the glucuronide, indicating sulfation as the predominant metabolic pathway. Despite extensive metabolism, hydroxytyrosol remained in plasma for up to 8 h with AUCs of 4293 and 8919 min·nmol/L for the doses of 3.85 and 7.70 g/kg, respectively. Therefore, table olives provide a more sustained plasma profile than other foods containing hydroxytyrosol, which may enhance its health-protecting activities. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Pharmacokinetics_of_Hydroxytyrosol_and_Its_Sulfate_and_Glucuronide_Metabolites_after_the_Oral_Administration_of_Table_Olives_to_Sprague-Dawley_Rats/25015992Test |
| DOI: | 10.1021/acs.jafc.3c06431.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jafc.3c06431.s001Test https://figshare.com/articles/journal_contribution/Pharmacokinetics_of_Hydroxytyrosol_and_Its_Sulfate_and_Glucuronide_Metabolites_after_the_Oral_Administration_of_Table_Olives_to_Sprague-Dawley_Rats/25015992Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.E05EB628 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jafc.3c06431.s001 |
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