دورية أكاديمية
RNA Interference-Screening of Potentially Lethal Gene Targets in the White-Backed Planthopper Sogatella furcifera via a Spray-Induced and Nanocarrier-Delivered Gene Silencing System
| العنوان: | RNA Interference-Screening of Potentially Lethal Gene Targets in the White-Backed Planthopper Sogatella furcifera via a Spray-Induced and Nanocarrier-Delivered Gene Silencing System |
|---|---|
| المؤلفون: | Yun-Feng Ma, Ting-Ting Liu, Ya-Qin Zhao, Juan Luo, Hong-Yan Feng, Yang-Yuntao Zhou, Lang-Lang Gong, Meng-Qi Zhang, Yin-yin He, J. Joe Hull, Youssef Dewer, Ming He, Peng He |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Microbiology, Cell Biology, Genetics, Molecular Biology, Immunology, Developmental Biology, Infectious Diseases, Plant Biology, Computational Biology, Space Science, Biological Sciences not elsewhere classified, transcriptional silencing mechanism, evaluate phenotypic effects, 2 days postspraying, two gene orthologs, identifying genes involved, delivered gene silencing, specific genes targeted, incorporated gene, target genes, α ,<, widespread post, wbph growth, wbph control, wbph ), transcript levels, sustainable biopesticide, specific degradation, significantly reduced, rna interference |
| الوصف: | RNA interference (RNAi) is a widespread post-transcriptional silencing mechanism that targets homologous mRNA sequences for specific degradation. An RNAi-based pest management strategy is target-specific and considered a sustainable biopesticide. However, the specific genes targeted and the efficiency of the delivery methods can vary widely across species. In this study, a spray-induced and nanocarrier-delivered gene silencing (SI-NDGS) system that incorporated gene-specific dsRNAs targeting conserved genes was used to evaluate phenotypic effects in white-backed planthopper (WBPH). At 2 days postspraying, transcript levels for all target genes were significantly reduced and knockdown of two gene orthologs, hsc70-3 and PP-α, resulted in an elevated mortality (>60%) and impaired ecdysis. These results highlight the utility of the SI-NDGS system for identifying genes involved in WBPH growth and development that could be potentially exploitable as high mortality target genes to develop an alternative method for WBPH control. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/RNA_Interference-Screening_of_Potentially_Lethal_Gene_Targets_in_the_White-Backed_Planthopper_Sogatella_furcifera_via_a_Spray-Induced_and_Nanocarrier-Delivered_Gene_Silencing_System/24935708Test |
| DOI: | 10.1021/acs.jafc.3c05659.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jafc.3c05659.s001Test https://figshare.com/articles/journal_contribution/RNA_Interference-Screening_of_Potentially_Lethal_Gene_Targets_in_the_White-Backed_Planthopper_Sogatella_furcifera_via_a_Spray-Induced_and_Nanocarrier-Delivered_Gene_Silencing_System/24935708Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.206FC069 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jafc.3c05659.s001 |
|---|