التفاصيل البيبلوغرافية
| العنوان: |
Site-Specific Conjugation Quantitation of a Cysteine-Conjugated Antibody–Drug Conjugate Using Stable Isotope Labeling Peptide Mapping LC–MS/MS Analysis |
| المؤلفون: |
Tyler K. Davis (12028410), Mark E. Jennings (10312270) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Physiology, Pharmacology, Biotechnology, Chemical Sciences not elsewhere classified, provide “ bottom, orthogonal analytical methods, drug conjugation sites, determine global drug, critical quality attribute, attribute analytical method, specific conjugation levels, lc – ms, specific conjugation quantitation, ” dar characterization, provide complementary site, ms analysis drug, specific quantitation, translational modification, protein sequence, one multi, mam ), correlate well, conjugated adc, antibody ratios, affecting potency, accurately quantitate |
| الوصف: |
Drug-load (DL) characterization of antibody–drug conjugates (ADCs) is an important analytical task due to its designation as a critical quality attribute (CQA) affecting potency and stability. Intact and subunit liquid chromatography–mass spectrometry (LC–MS) analyses can determine global drug-to-antibody ratios (DARs) that correlate well with other orthogonal analytical methods; however, peptide mapping liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis has struggled to provide complementary site-specific quantitation of drug conjugation sites. The peptide mapping method described herein utilizes stable isotope labeling to accurately quantitate the site-specific conjugation levels of a cysteine-conjugated ADC to provide “bottom-up” DAR characterization in parallel with protein sequence and post-translational modification (PTM) characterization in one multi-attribute analytical method (MAM). |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Site-Specific_Conjugation_Quantitation_of_a_Cysteine-Conjugated_Antibody_Drug_Conjugate_Using_Stable_Isotope_Labeling_Peptide_Mapping_LC_MS_MS_Analysis/19102491Test |
| DOI: |
10.1021/acs.analchem.1c04025.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.analchem.1c04025.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.F911FFB8 |
| قاعدة البيانات: |
BASE |
