دورية أكاديمية
The Mechanism and Potential Therapeutic Effects of Cyclosporin, Cyclophilin, Probiotics and Syndecan-1 in an Animal Model of Inflammatory Bowel Disease
| العنوان: | The Mechanism and Potential Therapeutic Effects of Cyclosporin, Cyclophilin, Probiotics and Syndecan-1 in an Animal Model of Inflammatory Bowel Disease |
|---|---|
| المؤلفون: | Dosh L., Rappa F., Jurjus A., Karam G., Lezeik R., El Masri J., Bucchieri F., Leone A., Jurjus R. |
| المساهمون: | Dosh L., Rappa F., Jurjus A., Karam G., Lezeik R., El Masri J., Bucchieri F., Leone A., Jurjus R. |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute (MDPI) |
| سنة النشر: | 2024 |
| المجموعة: | IRIS Università degli Studi di Palermo |
| مصطلحات موضوعية: | cyclophilin A, cyclosporine A,IBD, inflammation, probiotics, syndecan-1, Settore BIO/16 - Anatomia Umana |
| الوصف: | Background: Inflammatory bowel diseases (IBDs) have several treatment modalities including immunoregulators, like cyclosporine A, an immunosuppressant that interacts with cytoplasmic cyclophilin A, and probiotics. Aims: This study explored and compared the possible role of syndecan-1 in the IBD pathogenic process as well as the effectiveness of cyclophilin A, cyclosporine A, and their combination in the management of IBDs in the presence of probiotics. Methodology: IBD was induced in a total of 112 mice equally divided between syndecan-1 knock-out (KO) and Balb/c wild-type mice, using 2% dextran sulfate sodium (DSS) followed by intraperitoneal treatment with cyclosporine A, cyclophilin A, or a combination of both. In addition, a daily dose of probiotics was given in their drinking water. The animals were monitored for clinical signs and symptoms and checked for gross pathologies in the abdomen after 3 weeks. Descending and sigmoid colon biopsies were collected and fixed for routine microscopy or frozen for protein extraction and molecular testing for IL-6, CD3, CD147, and beta 1 integrins as well as pAkt expression. Results: The data showed that the induction of IBD in the syndecan-1 KO mice was more severe at the clinical, histological, and molecular levels than in the wild type. The combined CypA-CyA treatment showed no added inhibitory effect compared to single-drug treatment in both strains. Probiotics added to the combination was more effective in the wild type and, when used alone, its inhibition of IL-6 was the highest. As for the CD147 marker, there were more suppressions across the various groups in the KO mice except for the probiotics-alone group. Concerning CD3, it was significantly increased by the CypA-CyA complex, which led to more inflammation in the KO mice. Probiotics had little effect with the combination. In relation to beta 1 integrins, the CypA-CyA combination made no significant difference from CyA alone, and adding probiotics to the combination resulted in higher beta 1 integrin ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/38276500; volume:16; issue:1; firstpage:1; lastpage:21; numberofpages:21; journal:PHARMACEUTICS; https://hdl.handle.net/10447/622954Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85183192180 |
| DOI: | 10.3390/pharmaceutics16010130 |
| الإتاحة: | https://doi.org/10.3390/pharmaceutics16010130Test https://hdl.handle.net/10447/622954Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.AF03B9EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/pharmaceutics16010130 |
|---|