Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata
| العنوان: | Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata |
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| المؤلفون: | Bashar Khiatah, Shiela Bilbao, Jeffrey Rawson, Amber Tucker, Fouad Kandeel, Elena Forouhar, Kuan-Tsen Chen, Keiko Omori, Meirigeng Qi, Luis Valiente, Leonard Medrano, Ismail H. Al-Abdullah, Rachel Perez |
| المصدر: | Islets |
| بيانات النشر: | Informa UK Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, Cell Survival, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Islets of Langerhans Transplantation, Thermolysin, Cell Separation, Mice, SCID, collagenase gold, 030230 surgery, Islets of Langerhans, Mice, Young Adult, 03 medical and health sciences, Oxygen Consumption, 0302 clinical medicine, Endocrinology, Liberase MTF C/T, Mice, Inbred NOD, Neutral protease, islet isolation, medicine, Animals, Humans, Collagenases, Retrospective Studies, chemistry.chemical_classification, geography, geography.geographical_feature_category, Protease, Chemistry, Graft Survival, Middle Aged, Islet, medicine.anatomical_structure, Enzyme, Biochemistry, neutral protease, collagenase NB1, Collagenase, 030211 gastroenterology & hepatology, Digestion, Pancreas, Research Paper, Peptide Hydrolases, medicine.drug |
| الوصف: | Selection of enzymes for optimal pancreas digestion is essential for successful human islet isolations. The aim of this study was to evaluate the efficacy and outcome of using Collagenase Gold plus BP protease (VitaCyte) (n = 8) by comparing it to two commercially available enzymes, Liberase MTF C/T (Roche) (n = 48) and Collagenase NB1/NP (Serva) (n = 15). The isolation outcomes were assessed by islet counting, viability, glucose-stimulated oxygen consumption rate (OCR), and successful graft-rate following transplantation in diabetic NOD scid mice. The pancreas donor characteristics were not significantly different between the tested enzyme groups regarding their BMI, pancreas weight, cold ischemia time (CIT) and HbA1c. The results show that digested tissue volume was not statistically significant between the VitaCyte enzyme (34.25 ± 5.4 mL) and the Roche enzyme (55.25 ± 3.42 mL, p = 0.073), however, this was significant with Serva enzyme (64.07 ± 7.95 mL, p = 0.020). Interestingly, the islet yields were not statistically different between all enzyme groups. Moreover, when islets were transplanted into NOD scid mice, the reversal rate of diabetes for the VitaCyte enzyme group was similar to all enzyme groups. In conclusion, the effectiveness of Collagenase Gold plus BP protease is comparable to the MTF C/T and the Collagenase NB1/NP enzymes; the low cost could facilitate the use of more pancreata for islet isolations. |
| تدمد: | 1938-2022 1938-2014 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c66ec8f79fb701ba15a4b61f2146d58Test https://doi.org/10.1080/19382014.2017.1417716Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3c66ec8f79fb701ba15a4b61f2146d58 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19382022 19382014 |
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