التفاصيل البيبلوغرافية
| العنوان: |
Notable Improvement of 3‑Hydroxypropionic Acid and 1,3-Propanediol Coproduction Using Modular Coculture Engineering and Pathway Rebalancing |
| المؤلفون: |
Yufei Zhang (509147), Hossain M. Zabed (8477328), Junhua Yun (9771506), Guoyan Zhang (2203600), Yang Wang (5921), Xianghui Qi (8477334) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Molecular Biology, Pharmacology, Biotechnology, Immunology, Space Science, Chemical Sciences not elsewhere classified, coli coculture systems, coculture engineering, glycerol consumption, approach, PDOR, Pathway Rebalancing Sustainable bio., Modular Coculture Engineering, PDO, 3- HP, gabD 4 gene, UTR, HPA, Escherichia coli strains |
| الوصف: |
Sustainable bioconversion of glycerol into 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) is often bottlenecked by the accumulation of a toxic metabolic intermediate, 3-hydroxypropanaldehyde (3-HPA), mostly due to the imbalanced expression of the two key enzymes, namely, aldehyde dehydrogenase (AldH) and 1,3-PDO oxidoreductase (PDOR). Herein, we attempted to overcome it by modular coculture engineering using Lactobacillus reuteri and recombinant Escherichia coli strains through overexpressing an AldH (GabD4) and a PDOR (PduQ) in E. coli and pathway rebalancing via 5′-untranslated region (5′-UTR) engineering in the gabD4 gene. We then used the L. reuteri – E. coli coculture systems in resting cell biocatalysis of glycerol under several technological configurations to produce 3-HP and 1,3-PDO. The conventional approach, which is a two-step process comprising resting cell production (first step) and biotransformation of glycerol with resting cells (second step), produced 51.36 g/L 3-HP and 34.27 g/L 1,3-PDO from 120 g/L glycerol, leaving more than 30 g/L glycerol unconverted. Subsequently, we did experiments in a modified approach, including one more biotransformation step with the two-step approach to convert the residual glycerol. This three-step approach increased glycerol consumption to 140 g/L and produced 71.62 g/L 3-HP and 47.68 g/L 1,3-PDO. Finally, the sequential feeding of glycerol and fresh cells notably improved glycerol consumption to 240 g/L and titers of 3-HP and 1,3-PDO to 125.93 and 88.46 g/L, respectively, with a net titer of 214.39 g/L, which was the highest titer ever reported. Therefore, modular coculture engineering with an appropriate technological configuration could have the potential for improved production of value-added chemicals, particularly 3-HP and 1,3-PDO. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Notable_Improvement_of_3_Hydroxypropionic_Acid_and_1_3-Propanediol_Coproduction_Using_Modular_Coculture_Engineering_and_Pathway_Rebalancing/14233198Test |
| DOI: |
10.1021/acssuschemeng.1c00229.s001 |
| الإتاحة: |
https://doi.org/10.1021/acssuschemeng.1c00229.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.9E598B2C |
| قاعدة البيانات: |
BASE |
