التفاصيل البيبلوغرافية
| العنوان: |
A Transfer Hydrogenation Approach to Activity-Based Sensing of Formate in Living Cells |
| المؤلفون: |
Steven W. M. Crossley, Logan Tenney, Vanha N. Pham, Xiao Xie, Michelle W. Zhao, Christopher J. Chang |
| سنة النشر: |
2024 |
| المجموعة: |
Smithsonian Institution: Figshare |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Molecular Biology, Pharmacology, Cancer, Inorganic Chemistry, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, various fluorophore scaffolds, color ratiometric response, transfer hydrogenation approach, nucleic acid biosynthesis, enable fluorescence detection, human colon cancer, component probe system, based sensing system, endogenous formate fluxes, living cells formate, cell formate imaging, living cells, based sensing, formate detection, endogenous precursor, chemical approach, amino acid, based models, formate levels, formate facilitates, cellular formate |
| الوصف: |
Formate is a major reactive carbon species in one-carbon metabolism, where it serves as an endogenous precursor for amino acid and nucleic acid biosynthesis and a cellular source of NAD(P)H. On the other hand, aberrant elevations in cellular formate are connected to progression of serious diseases, including cancer and Alzheimer’s disease. Traditional methods for formate detection in biological environments often rely on sample destruction or extensive processing, resulting in a loss of spatiotemporal information. To help address these limitations, here we present the design, synthesis, and biological evaluation of a first-generation activity-based sensing system for live-cell formate imaging that relies on iridium-mediated transfer hydrogenation chemistry. Formate facilitates an aldehyde-to-alcohol conversion on various fluorophore scaffolds to enable fluorescence detection of this one-carbon unit, including through a two-color ratiometric response with internal calibration. The resulting two-component probe system can detect changes in formate levels in living cells with a high selectivity over potentially competing biological analytes. Moreover, this activity-based sensing system can visualize changes in endogenous formate fluxes through alterations of one-carbon pathways in cell-based models of human colon cancer, presaging the potential utility of this chemical approach to probe the continuum between one-carbon metabolism and signaling in cancer and other diseases. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/A_Transfer_Hydrogenation_Approach_to_Activity-Based_Sensing_of_Formate_in_Living_Cells/25392326Test |
| DOI: |
10.1021/jacs.3c09735.s001 |
| الإتاحة: |
https://doi.org/10.1021/jacs.3c09735.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.A2D31FC5 |
| قاعدة البيانات: |
BASE |
