Bruguiera gymnorhiza hypocotyl is a common marine herbal medicine of the Jing people used in the treatment of hepatitis B. Firstly, MTT and real-time fluorescence quantitative PCR methods were used to determine the anti-HBV activity of different extracted parts of B. gymnorhiza hypocotyl, the chemical constituents of the active extraction parts were isolated and identified using modern chromatographic techniques and spectroscopic methods, and in vitro screening model was employed to test the anti-HBV activity of obtained compounds. The results were as follows: (1) The n-butanol phase of the hypocotyl exhibited anti-HBV activity; (2) A total of 11 compounds were isolated and structurally identified, namely uridine (1), thymidine (2), adenosine (3), oryzalactam (4), n-butyl-O-D-fructopyranoside (5), nortetillapyrone (6), (4R,6S)-4-methoxyl-2,3-dihydroaquilegiolide (7), (4R,6S)-2-dihydromenisdaurilide (8), gallcatechin (9), 1-(4-hydroxy-3-methoxy)-phenyl-2-［4-(1,2,3-trihydroxypropyl)-2-methoxy］-phenoxy-1,3-propandiol(10), and (-)-lyoniresinol-9-O-β-D-xylopyrano-side (11), among them, compounds 4, 5, 7 and 8 were firstly obtained from B. gymnorhiza, and Compound 4 showed anti-HBV activity with an inhibition rate of 23.59%. The study clarify the chemical composition of the anti-HBV of B. gymnorhiza hypocotyl.
