التفاصيل البيبلوغرافية
| العنوان: |
Near-Infrared Small Molecule as a Specific Fluorescent Probe for Ultrasensitive Recognition of Antiparallel Human Telomere G‑Quadruplexes |
| المؤلفون: |
Ming Jin (770244), Jing Li (10611), Yang Chen (9737), Jie Zhao (49409), Jiahui Zhang (3163929), Zhen Zhang (86004), Peiyao Du (8849297), Libing Zhang (1481890), Xiaoquan Lu (1427404) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Neuroscience, Biotechnology, Cancer, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, sensitivity, probe, selectivity, nm, TOVJ, telomere G 4 detection, DNA, fluorescence, antiparallel G 4s, G 4s limit, G 4s, 22 AG Na |
| الوصف: |
In the past 10 years, many fluorescent probes have been developed to recognize G-quadruplexes (G4s) since G4s play an important role in biological systems. However, the selectivity and sensitivity of existing probes for G4s limit their further applications. Herein, we design and synthesize a new probe (TOVJ) by introducing 9-vinyljulolidine into TO. The new probe exhibits almost no fluorescence in an aqueous solution. Upon interacting with G4s, especially the antiparallel G4s, the fluorescence intensity was greatly enhanced (maximum 2742-fold) with a large Stokes shift of 198 nm and the maximum emission peak at 694 nm (near-infrared region). TOVJ showed high sensitivity and selectivity to G4s over other DNA topologies (ssDNA/dsDNA), especially to antiparallel G4s. For antiparallel human telomere G4 detection, the limits of detection of Hum24 and 22AG Na + were as low as 164 and 231 pM, respectively. This indicates that TOVJ is a highly sensitive fluorescence sensor that can be effectively used for antiparallel human telomere G4 detection. The result of live-cell imaging showed that TOVJ could enter live cells and locate in the mitochondria. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Near-Infrared_Small_Molecule_as_a_Specific_Fluorescent_Probe_for_Ultrasensitive_Recognition_of_Antiparallel_Human_Telomere_G_Quadruplexes/14916671Test |
| DOI: |
10.1021/acsami.1c07101.s001 |
| الإتاحة: |
https://doi.org/10.1021/acsami.1c07101.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.5B905B12 |
| قاعدة البيانات: |
BASE |
