Identification of AcnR, a TetR-type Repressor of the Aconitase Gene acn in Corynebacterium glutamicum

التفاصيل البيبلوغرافية
العنوان: Identification of AcnR, a TetR-type Repressor of the Aconitase Gene acn in Corynebacterium glutamicum
المؤلفون: Volker F. Wendisch, Andreas Krug, Michael Bott
المصدر: The journal of biological chemistry 585-595 (2005). doi:10.1074/jbc.M408271200
بيانات النشر: Elsevier BV, 2005.
سنة النشر: 2005
مصطلحات موضوعية: Transcriptional Activation, acetate metabolism, metabolism [Bacterial Proteins], genetics [Aconitate Hydratase], Molecular Sequence Data, Repressor, binding protein, posttranscriptional regulation, Biology, Biochemistry, Aconitase, nucleotide-sequence, Corynebacterium glutamicum, chemistry.chemical_compound, Species Specificity, Bacterial Proteins, ddc:570, RNA polymerase, Genes, Regulator, Transcriptional regulation, molecular analysis, TetR, Amino Acid Sequence, Molecular Biology, Gene, metabolism [Repressor Proteins], DNA microarray analyses, Aconitate Hydratase, enzymology [Corynebacterium glutamicum], respiratory-chain, genetics [Corynebacterium glutamicum], antagonists & inhibitors [Aconitate Hydratase], Gene Expression Regulation, Bacterial, Cell Biology, gel-electrophoresis, Molecular biology, Repressor Proteins, Citric acid cycle, genetics [Repressor Proteins], escherichia-coli aconitases, chemistry, Genes, Bacterial, bacillus-subtilis aconitase, Sequence Alignment, genetics [Bacterial Proteins]
الوصف: In Corynebacterium glutamicum, the activity of aconitase is 2.5-4-fold higher on propionate, citrate, or acetate than on glucose. Here we show that this variation is caused by transcriptional regulation. In search for putative regulators, a gene (acnR) encoding a TetR-type transcriptional regulator was found to be encoded immediately downstream of the aconitase gene (acn) in C. glutamicum. Deletion of the acnR gene led to a 5-fold increased acn-mRNA level and a 5-fold increased aconitase activity, suggesting that AcnR functions as repressor of acn expression. DNA microarray analyses indicated that acn is the primary target gene of AcnR in the C. glutamicum genome. Purified AcnR was shown to be a homodimer, which binds to the acn promoter in the region from -11 to -28 relative to the transcription start. It thus presumably acts by interfering with the binding of RNA polymerase. The acn-acnR organization is conserved in all corynebacteria and mycobacteria with known genome sequence and a putative AcnR consensus binding motif (CAGNACnnncGTACTG) was identified in the corresponding acn upstream regions. Mutations within this motif inhibited AcnR binding. Because the activities of citrate synthase and isocitrate dehydrogenase were previously reported not to be increased during growth on acetate, our data indicate that aconitase is a major control point of tricarboxylic acid cycle activity in C. glutamicum, and they identify AcnR as the first transcriptional regulator of a tricarboxylic acid cycle gene in the Corynebacterianeae.
تدمد: 0021-9258
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::826b681b2a8d90478580768226c7a189Test
https://doi.org/10.1074/jbc.m408271200Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....826b681b2a8d90478580768226c7a189
قاعدة البيانات: OpenAIRE