دورية أكاديمية
Graphene Oxide Decreases Pro-Inflammatory Proteins Production in Skeletal Muscle Cells Exposed to SARS-CoV-2 Spike Protein
| العنوان: | Graphene Oxide Decreases Pro-Inflammatory Proteins Production in Skeletal Muscle Cells Exposed to SARS-CoV-2 Spike Protein |
|---|---|
| المؤلفون: | Bałaban J, Wierzbicki M, Zielińska-Górska M, Sosnowska M, Daniluk K, Jaworski S, Koczoń P, Cysewski D, Chwalibog A, Sawosz E |
| المصدر: | Nanotechnology, Science and Applications, Vol Volume 16, Pp 1-18 (2023) |
| بيانات النشر: | Dove Medical Press, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medical technology LCC:Chemical technology |
| مصطلحات موضوعية: | muscle cells, sars-cov-2, virus spike protein s, graphene oxide, cytokine storm, ace2inf, Medical technology, R855-855.5, Chemical technology, TP1-1185 |
| الوصف: | Jaśmina Bałaban,1 Mateusz Wierzbicki,1 Marlena Zielińska-Górska,1 Malwina Sosnowska,1 Karolina Daniluk,1 Sławomir Jaworski,1 Piotr Koczoń,2 Dominik Cysewski,3 André Chwalibog,4 Ewa Sawosz1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, Warsaw, Poland; 3Clinical Research Centre, Medical University of Bialystok, Białystok, Poland; 4Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, DenmarkCorrespondence: André Chwalibog, University of Copenhagen, Groennegaardsvej 3, Frederiksberg, 1870, Denmark, Tel +45 40963573, Email ach@sund.ku.dkAim: The experiments aimed to document the presence of the ACE2 receptor on human muscle cells and the effects of the interaction of these cells with the spike protein of the SARS-CoV-2 virus in terms of induction of pro-inflammatory proteins, as well as to assess the possibility of reducing the pool of these proteins with the use of graphene oxide (GO) flakes.Methods: Human Skeletal Myoblast (HSkM), purchased from Gibco were maintained in standard condition according to the manufacturer’s instruction. The cells were divided into 4 groups; 1. C-control, 2. S-with addition of spike protein, 3. GO-with the addition of graphene oxide, 4. GO-S-with addition of GO followed by the addition of S protein. Protein S (PX-COV-P049) was purchased from ProteoGenix (France). GO was obtained from Advanced Graphene Products (Zielona Gora, Poland). The influence of all the factors on the morphology of cells was investigated using light and confocal microscopy. ACE2 protein expression on muscle cells was visualized and 40 pro-inflammatory cytokines were investigated using the membrane antibody array method. The protein profile of the lysate of cells from individual groups was also analyzed by mass spectrometry.Conclusion: The experiments confirmed the presence of the ACE2 receptor in human skeletal muscle cells. It has also been documented that the SARS-CoV-2 virus spike protein influences the activation of selected pro-inflammatory proteins that promote cytokine storm and oxidative stress in muscle cells. The use of low levels of graphene oxide does not adversely affect muscle cells, reducing the levels of most proteins, including pro-inflammatory proteins. It can be assumed that GO may support anti-inflammatory therapy in muscles by scavenging proteins that activate cytokine storm.Keywords: muscle cells, SARS-CoV-2, virus spike protein S, graphene oxide, cytokine storm, ACE2 |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1177-8903 |
| العلاقة: | https://www.dovepress.com/graphene-oxide-decreases-pro-inflammatory-proteins-production-in-skele-peer-reviewed-fulltext-article-NSATest; https://doaj.org/toc/1177-8903Test |
| الوصول الحر: | https://doaj.org/article/a6335dda070a488bb6ac548115eb3647Test |
| رقم الانضمام: | edsdoj.6335dda070a488bb6ac548115eb3647 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11778903 |
|---|