دورية أكاديمية
International study on inter-reader variability for circulating tumor cells in breast cancer
| العنوان: | International study on inter-reader variability for circulating tumor cells in breast cancer |
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| المؤلفون: | Ignatiadis, M. (Michael), Riethdorf, S. (Sabine), Bidard, F.-C. (François-Clement), Vaucher, I. (Isabelle), Khazour, M. (Mustapha), Rothé, F. (Françoise), Metallo, J. (Jessica), Rouas, G. (Ghizlane), Payne, R.E. (Rachel), Coombes, R.C. (Raoul), Teufel, I. (Ingrid), Andergassen, U. (Ulrich), Apostolaki, M. (Maria), Politaki, E. (Eleni), Mavroudis, D. (Dimitris), Bessi, E. (Elena), Pestrin, M. (Marta), Di Leo, A. (Angelo), Campion, D. (Dominique), Reinholz, M. (Monica), Perez, E. (Edith), Piccart, M.J. (Martine), Borgen, E. (Elin), Naume, B. (Bjorn), Jimenez, J. (Jose), Aura, C.M. (Claudia), Zorzino, L. (Laura), Cassatella, M.C. (Maria), Sandri, M.T. (Maria), Mostert, B. (Bianca), Sleijfer, S. (Stefan), Kraan, J. (Jaco), Janni, W. (Wolfgang), Fehm, T. (Tanja), Rack, B. (Brigitte), Terstappen, L.W.M.M. (Leon), Repollet, M. (Madeline), Pierga, J.Y. (Jean Yves), Miller, C. (Craig), Sotiriou, C. (Christos), Michiels, S. (Stefan), Pantel, K. (Klaus) |
| المصدر: | Breast Cancer Research (Print) vol. 16 no. 2 |
| سنة النشر: | 2014 |
| المجموعة: | RePub - Publications from Erasmus University, Rotterdam |
| الوصف: | Introduction: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.Methods: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.Results: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P < 0.001) and from M0 and <3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P < 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90).Conclusions: The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://repub.eur.nl/pub/55184Test; urn:hdl:1765/55184 |
| DOI: | 10.1186/bcr3647 |
| الإتاحة: | https://doi.org/10.1186/bcr3647Test http://repub.eur.nl/pub/55184Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A516FF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/bcr3647 |
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