التفاصيل البيبلوغرافية
| العنوان: |
Longitudinal analysis of host protein serum signatures of treatment and recovery in pulmonary tuberculosis |
| المؤلفون: |
Powell, Samantha M., Jarsberg, Leah G., Zionce, Erin L. M., Anderson, Lindsey N., Gritsenko, Marina A., Nahid, Payam, Jacobs, Jon M. |
| المساهمون: |
Tian, Suyan, National Institute of Health, National Institute of Allergy and Infectious Diseases, National Institute of Health, National Institute for Allergy and Infectious Diseases |
| المصدر: |
PLOS ONE ; volume 19, issue 2, page e0294603 ; ISSN 1932-6203 |
| بيانات النشر: |
Public Library of Science (PLoS) |
| سنة النشر: |
2024 |
| المجموعة: |
PLOS Publications (via CrossRef) |
| الوصف: |
Background A better understanding of treatment progression and recovery in pulmonary tuberculosis (TB) infectious disease is crucial. This study analyzed longitudinal serum samples from pulmonary TB patients undergoing interventional treatment to identify surrogate markers for TB-related outcomes. Methods Serum that was collected at baseline and 8, 17, 26, and 52 weeks from 30 TB patients experiencing durable cure were evaluated and compared using a sensitive LC-MS/MS proteomic platform for the detection and quantification of differential host protein signatures relative to timepoint. The global proteome signature was analyzed for statistical differences across the time course and between disease severity and treatment groups. Results A total of 676 proteins showed differential expression in the serum over these timepoints relative to baseline. Comparisons to understand serum protein dynamics at 8 weeks, treatment endpoints at 17 and 26 weeks, and post-treatment at 52 weeks were performed. The largest protein abundance changes were observed at 8 weeks as the initial effects of antibiotic treatment strongly impacted inflammatory and immune modulated responses. However, the largest number of proteome changes was observed at the end of treatment time points 17 and 26 weeks respectively. Post-treatment 52-week results showed an abatement of differential proteome signatures from end of treatment, though interestingly those proteins uniquely significant at post-treatment were almost exclusively downregulated. Patients were additionally stratified based upon disease severity and compared across all timepoints, identifying 461 discriminating proteome signatures. These proteome signatures collapsed into discrete expression profiles with distinct pathways across immune activation and signaling, hemostasis, and metabolism annotations. Insulin-like growth factor (IGF) and Integrin signaling maintained a severity signature through 52 weeks, implying an intrinsic disease severity signature well into the post-treatment ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1371/journal.pone.0294603 |
| الإتاحة: |
https://doi.org/10.1371/journal.pone.0294603Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.10266E01 |
| قاعدة البيانات: |
BASE |
