التفاصيل البيبلوغرافية
| العنوان: |
Table_1_Production of CFTR-ΔF508 Rabbits.DOCX |
| المؤلفون: |
Dongshan Yang (351679), Xiubin Liang (477089), Brooke Pallas (10019294), Mark Hoenerhoff (3171744), Zhuoying Ren (10019297), Renzhi Han (276195), Jifeng Zhang (352541), Y. Eugene Chen (8749887), Jian-Ping Jin (750064), Fei Sun (102854), Jie Xu (34477) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Genetics, Genetic Engineering, Biomarkers, Developmental Genetics (incl. Sex Determination), Epigenetics (incl. Genome Methylation and Epigenomics), Gene Expression (incl. Microarray and other genome-wide approaches), Genome Structure and Regulation, Genomics, Genetically Modified Animals, Livestock Cloning, Gene and Molecular Therapy, CRISPR/Cas9, cystic fbrosis, CFTR-ΔF508, rabbits, gene edit |
| الوصف: |
Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). The most common mutation is the deletion of phenylalanine residue at position 508 (ΔF508). Here we report the production of CFTR-ΔF508 rabbits by CRISPR/Cas9-mediated gene editing. After microinjection and embryo transfer, 77 kits were born, of which five carried the ΔF508 mutation. To confirm the germline transmission, one male ΔF508 founder was bred with two wild-type females and produced 16 F1 generation kits, of which six are heterozygous ΔF508/WT animals. Our work adds CFTR-ΔF508 rabbits to the toolbox of CF animal models for biomedical research. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/dataset/Table_1_Production_of_CFTR-_F508_Rabbits_DOCX/13624970Test |
| DOI: |
10.3389/fgene.2020.627666.s001 |
| الإتاحة: |
https://doi.org/10.3389/fgene.2020.627666.s001Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.FE9312B9 |
| قاعدة البيانات: |
BASE |
