التفاصيل البيبلوغرافية
| العنوان: |
Identification and map-based cloning of an EMS-induced mutation in wheat gene TaSP1 related to spike architecture. |
| المؤلفون: |
Zhang, Lin, Zhou, Huidan, Fu, Xian, Zhou, Niuniu, Liu, Mengjie, Bai, Shenglong, Zhao, Xinpeng, Cheng, Ruiru, Li, Suoping, Zhang, Dale |
| المصدر: |
Theoretical & Applied Genetics; Jun2024, Vol. 137 Issue 6, p1-11, 11p |
| مصطلحات موضوعية: |
MOLECULAR cloning, RECESSIVE genes, GENE mapping, GENETIC mutation, HAPLOTYPES, WHEAT |
| مستخلص: |
Key message: A candidate gene TaSP1 related to spike shape was cloned, and the gene-specific marker was developed to efficiently track the superior haplotype in common wheat. Spike shape, an important factor that affects wheat grain yield, is mainly defined by spike length (SPL), spikelet number (SPN), and compactness. Zhoumai32 mutant 1160 (ZM1160), a mutant obtained from ethyl methane sulfonate (EMS) treatment of hexaploid wheat variety Zhoumai32, was used to identify and clone the candidate gene that conditioned the spike shape. Genetic analysis of an F2 population derived from a cross of ZM1160 and Bainong207 suggested that the compact spike shape in ZM1160 was controlled by a single recessive gene, and therefore, the mutated gene was designated as Tasp1. With polymorphic markers identified through bulked segregant analysis (BSA), the gene was mapped to a 2.65-cM interval flanked by markers YZU0852 and MIS46239 on chromosome 7D, corresponding to a 0.42-Mb physical interval of Chinese spring (CS) reference sequences (RefSeq v1.0). To fine map TaSP1, 15 and seven recombinants were, respectively, screened from 1599 and 1903 F3 plants derived from the heterozygous F2 plants. Finally, TaSP1 was delimited to a 21.9 Kb (4,870,562 to 4,892,493 bp) Xmis48123-Xmis48104 interval. Only one high-confidence gene TraesCS7D02G010200 was annotated in this region, which encodes an unknown protein with a putative vWA domain. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that TraesCS7D02G010200 was mainly expressed in the spike. Haplotype analysis of 655 wheat cultivars using the candidate gene-specific marker Xg010200p2 identified a superior haplotype TaSP1b with longer spike and more spikelet number. TaSP1 is beneficial to the improvement in wheat spike shape. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
