دورية أكاديمية
Reproducibility of fluorescent expression from engineered biological constructs in E. coli
| العنوان: | Reproducibility of fluorescent expression from engineered biological constructs in E. coli |
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| المؤلفون: | Beal, J., Haddock-Angelli, T., Gershater, M., De Mora, K., Lizarazo, M., Hollenhorst, J., Rettberg, R., Demling, P., Hanke, R., Osthege, M., Schechtel, A., Sudarsan, S., Zimmermann, A., Gabryelczyk, B., Ikonen, M., Salmela, M., Acar, M., Aktas, M. F., Bestepe, F., Ceylan, F. S., Cigdem, S., Dohan, M., Elitok, M., Gunduz, M., Gunduz, E., Hatipoglu, O. F., Kaya, T., Sayin, O., Tapan, S., Tereci, O. F., Uçar, A., Yilmaz, M., Barrick, J., Gutierrez, A., Mishler, D., Monk, J., Mortensen, K., Shin, N., Watkins, E., Chen, Y., Jin, Y., Shi, Y., Zhang, H. M., Ono, B., Paino, I. M. M., Ribovski, L., Silva, I., Zampronio, D. K., Birkholz, N., Busche, R. F., Konzock, O., Lippold, S., Ludwig, C., Philippi, M., Platz, L., Sigismund, C., Weber, S., Wehrs, M., Werchau, N., Wronska, A., Yen, Z. Z., Agarwal, Y., Appleton, E., Densmore, D., Esmurria, A., Lewis, K., Pacheco, A., Bruchez, M., Peters, D., Telmer, C., Wang, L., Canas-Duarte, S., Giraldo-Perez, D., Gomez-Garzon, C., Madrid-Wolff, J., Marin-Medina, N., Mazzanti, V., Rodriguez-Forero, L., Scher, E., Dowell, R., O'Hara, S., Pogoda, C. S., Shattuck, K., Altintas, A., Bali, A. P., Bech, R., Egholm, A., Hansen, A. S. L., Jensen, K., Karlsen, K. B., Mosbech, C., Belkhelfa, S., Berenger, N., Bodinier, R., Jacry, C. |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2016 |
| المجموعة: | University of Leicester: Leicester Research Archive (LRA) |
| الوصف: | We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices. ; Raytheon BBN Technologies, Synthace, and Agilent provided support in the form of salaries for authors JB, MG, and JH, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; Peer-reviewed ; Publisher Version |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | PLoS ONE, 2016, 11(3): e0150182; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150182Test; http://hdl.handle.net/2381/38541Test |
| DOI: | 10.1371/journal.pone.0150182 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0150182Test http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150182Test http://hdl.handle.net/2381/38541Test |
| حقوق: | © 2016 Beal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| رقم الانضمام: | edsbas.718F60EA |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0150182 |