دورية أكاديمية
Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda
| العنوان: | Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda |
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| المؤلفون: | Keinprecht, Helga (University of Veterinary Medicine Vienna / New Vision Veterinary Hospital), Loncaric, Igor (University of Veterinary Medicine Vienna), Feßler, Andrea T. (Freie Universität Berlin), Desvars-Larrive, Amelie (University of Veterinary Medicine Vienna / Complexity Science Hub Vienna), Müller, Elke (Leibniz Institute of Photonic Technology / InfectoGnostics Research Campus), Wittek, Thomas (University of Veterinary Medicine Vienna), Zöchbauer, Jennifer (University of Veterinary Medicine Vienna), Szostak, Michael P. (University of Veterinary Medicine Vienna), Fischer, Otto W. (New Vision Veterinary Hospital), Marek, Lydia (University of Veterinary Medicine Vienna / New Vision Veterinary Hospital), Irimaso, Emmanuel (University of Rwanda), Rosel, Adriana Cabal (Austrian Agency for Health and Food Safety), Grunert, Tom (University of Veterinary Medicine Vienna), Stessl, Beatrix (University of Veterinary Medicine Vienna), Ntakirutimana, Christophe (New Vision Veterinary Hospital), Ruppitsch, Werner (Austrian Agency for Health and Food Safety), Ehricht, Ralf (Leibniz Institute of Photonic Technology / Friedrich Schiller University Jena), Monecke, Stefan (Leibniz Institute of Photonic Technology / Universitätsklinik Dresden), Ehling-Schulz, Monika (University of Veterinary Medicine Vienna), Spergser, Joachim (University of Veterinary Medicine Vienna), Schwarz, Stefan (Freie Universität Berlin), Braun, Sascha D. (Leibniz Institute of Photonic Technology / InfectoGnostics Research Campus) |
| المصدر: | Journal of Global Antimicrobial Resistance 36, 326-335 (2024) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Female, Cattle, Animals, Humans, Staphylococcus aureus, Rwandaepidemiology, Staphylococcal Infectionsepidemiologyveterinary, Anti-Bacterial Agentspharmacology, Mastitis |
| الوصف: | The objective of the present study was to examine the diversity of Staphylococcus aureus from mastitis milk samples of cows in Rwanda.A total of 1080 quarter milk samples from 279 dairy cows were collected in 80 different farms from all five provinces of Rwanda. In total, 135 S. aureus isolates were obtained and subjected to genotyping (spa typing, DNA microarray, whole-genome sequencing (WGS)), antimicrobial susceptibility testing (AST) and phenotypic profiling by Fourier Transform Infrared (FTIR) spectroscopy (including capsular serotyping).Resistance to penicillin and/or tetracycline was most frequently observed. Ten sequence types (STs) (ST1, ST151, ST152, ST5477, ST700, ST7110, ST7983, ST7984, ST8320, ST97) belonging to seven clonal complexes (CCs) (CC1, CC130, CC152, CC3591, CC3666, CC705, CC97) were detected. The Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83) and the human and bovine toxic shock syndrome toxin gene tst-1 variants were detected. FTIR-based capsular serotyping showed CC-specific differences. Most CC97 (cap5 allele) isolates were primarily nonencapsulated (82%), whereas isolates of CC3591 and CC3666 (cap8 allele) were mostly encapsulated (86.4% and 57.8%, respectively). Our results underline the widespread global distribution of cattle-adapted CC97.The presence of CC3591 and CC3666 in bovine mastitis suggests an important role in cattle health and dairy production in Rwanda. The results of the present study support the need for a rigorous One-Health Surveillance program of the bovine-human interface. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | isPartOf:https://phaidra.vetmeduni.ac.at/o:605Test[Publications / University of Veterinary Medicine Vienna]; https://phaidra.vetmeduni.ac.at/o:3082Test |
| DOI: | 10.1016/j.jgar.2024.01.017 |
| الإتاحة: | https://doi.org/10.1016/j.jgar.2024.01.017Test https://phaidra.vetmeduni.ac.at/o:3082Test |
| حقوق: | CC BY-NC-ND 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.99BF8C25 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jgar.2024.01.017 |
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