Diagnostic accuracy of the anti‐glutamic acid decarboxylase antibody in type 1 diabetes mellitus: Comparison between radioimmunoassay and enzyme‐linked immunosorbent assay
| العنوان: | Diagnostic accuracy of the anti‐glutamic acid decarboxylase antibody in type 1 diabetes mellitus: Comparison between radioimmunoassay and enzyme‐linked immunosorbent assay |
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| المؤلفون: | Noriko Satoh-Asahara, Yaeko Kawaguchi, Takashi Murata, Fumi Yoshioka, Yukako Mizutani, Naoki Sakane, Shinji Odori, Kazuhiko Kotani, Hayami Okada, Akira Shimatsu, Kokoro Tsuzaki, Shinsuke Nirengi, Tomokazu Watanabe, Masami Tsukamoto, Kazunori Yamada, Reiko Nakagawachi |
| المصدر: | Journal of Diabetes Investigation |
| بيانات النشر: | John Wiley and Sons Inc., 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Glutamate decarboxylase, Radioimmunoassay, Type 1 diabetes mellitus, 030209 endocrinology & metabolism, Diagnostic accuracy, Enzyme-Linked Immunosorbent Assay, 030204 cardiovascular system & hematology, Gastroenterology, Anti‐glutamic acid decarboxylase antibody, 03 medical and health sciences, 0302 clinical medicine, Diabetes mellitus, Internal medicine, Internal Medicine, medicine, Humans, Aged, chemistry.chemical_classification, Type 1 diabetes, biology, business.industry, Glutamate Decarboxylase, General Medicine, Articles, Middle Aged, medicine.disease, Molecular biology, Enzyme‐linked immunosorbent assay, Enzyme, Clinical Science and Care, Cross-Sectional Studies, Diabetes Mellitus, Type 1, chemistry, biology.protein, Original Article, Female, Antibody, business, Kappa |
| الوصف: | Aims/Introduction The distributer of the anti-glutamic acid decarboxylase antibody assay kit using radioimmunoassay (RIA) recently announced its discontinuation, and proposed an alternative kit using enzyme-linked immunosorbent assay (ELISA). The aim of the present study was to investigate the diagnostic values of the anti-glutamic acid decarboxylase antibody by RIA and ELISA among type 1 diabetes mellitus patients and control participants. Materials and Methods A total of 79 type 1 diabetes mellitus patients and 79 age-matched controls were enrolled and assessed using RIA and ELISA. Sensitivity, specificity, positive predictive values and negative predictive values were calculated for cut-off values (RIA = 1.5 U/mL and ELISA = 5.0 U/mL, respectively). Kappa coefficients were used to test for agreements between the RIA and ELISA methods regarding the diagnosis of type 1 diabetes mellitus. Results The sensitivity, specificity, positive predictive values, and negative predictive values for diagnosing type 1 diabetes mellitus were 57.0, 97.5, 95.7, and 69.4% by RIA, and 60.8, 100.0, 100.0 and 71.8% by ELISA, respectively. The diagnosis of type 1 diabetes mellitus using the RIA and ELISA methods showed substantial agreement with the kappa values of 0.74 for all participants, and of 0.64 for the acute type; however, there was moderate agreement with the kappa value of 0.56 for the slowly progressive type. Conclusions The present study suggests that both anti-glutamic acid decarboxylase antibody by RIA and ELISA was useful for diagnosing type 1 diabetes mellitus. However, in the slowly progressive type, the degree of agreement of these two kits was poorer compared with those in all participants or in the acute type. |
| اللغة: | English |
| تدمد: | 2040-1124 2040-1116 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4ea5c836509d97eb5b07fc2bd8f96d2Test http://europepmc.org/articles/PMC5497031Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f4ea5c836509d97eb5b07fc2bd8f96d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20401124 20401116 |
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