Transforming yeast peroxisomes into microfactories for the efficient production of high-value isoprenoids
| العنوان: | Transforming yeast peroxisomes into microfactories for the efficient production of high-value isoprenoids |
|---|---|
| المؤلفون: | Yixuan Liu, Sotirios C. Kampranis, Simon Dusséaux, Codruta Ignea, William Thomas Wajn |
| المصدر: | Dusséaux, S, Wajn, W T, Liu, Y, Ignea, C & Kampranis, S C 2020, ' Transforming yeast peroxisomes into microfactories for the efficient production of high-value isoprenoids ', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 50, pp. 31789-31799 . https://doi.org/10.1073/pnas.2013968117Test Proc Natl Acad Sci U S A |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, Mevalonate pathway, Mevalonic Acid, Saccharomyces cerevisiae, Terpenoid, Metabolic engineering, Industrial Microbiology, Peroxisomes, Intramolecular Lyases, Aldose-Ketose Isomerases, Synthetic biology, Plant Proteins, Indole test, Multidisciplinary, Chemistry, Terpenes, Peroxisome, Biological Sciences, Yeast, Diphosphates, Cytosol, Metabolic pathway, Biochemistry, Metabolic Engineering, Compartmentalization, Synthetic Biology, Diterpenes, Metabolic Networks and Pathways |
| الوصف: | Current approaches for the production of high-value compounds in microorganisms mostly use the cytosol as a general reaction vessel. However, competing pathways and metabolic cross-talk frequently prevent efficient synthesis of target compounds in the cytosol. Eukaryotic cells control the complexity of their metabolism by harnessing organelles to insulate biochemical pathways. Inspired by this concept, herein we transform yeast peroxisomes into microfactories for geranyl diphosphate-derived compounds, focusing on monoterpenoids, monoterpene indole alkaloids, and cannabinoids. We introduce a complete mevalonate pathway in the peroxisome to convert acetyl-CoA to several commercially important monoterpenes and achieve up to 125-fold increase over cytosolic production. Furthermore, peroxisomal production improves subsequent decoration by cytochrome P450s, supporting efficient conversion of (S)-(-)-limonene to the menthol precursor trans-isopiperitenol. We also establish synthesis of 8-hydroxygeraniol, the precursor of monoterpene indole alkaloids, and cannabigerolic acid, the cannabinoid precursor. Our findings establish peroxisomal engineering as an efficient strategy for the production of isoprenoids. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfee06238cd1190a81cc3654b11b13f6Test https://curis.ku.dk/ws/files/286842647/31789.full.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cfee06238cd1190a81cc3654b11b13f6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |