التفاصيل البيبلوغرافية
| العنوان: |
The Effect of Irradiated Riboflavin in Human Tenon’s Fibroblast – A Study on Cellular Viability |
| المؤلفون: |
Wendy Yen Nee See (11885236), Fazliana Ismail (11885239), Siti Hamimah Sheikh Abdul Kadir (3873847), Visvaraja Subrayan (11885242) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Medicine, Cell Biology, Genetics, Neuroscience, Pharmacology, Marine Biology, Cancer, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Riboflavin, irradiated riboflavin, cellular viability, human tenon fibroblast, MTT assay |
| الوصف: |
The main purpose of this work is to study the cellular viability effect of irradiated riboflavin in cultured human tenon fibroblasts. The tenon tissue was harvested from a patient undergoing strabismus surgery. The human tenon fibroblast cell culture and isolation were performed according to the standard laboratory cell culturing protocol. The cells were divided into three groups: control, treatment with irradiated and non-irradiated riboflavin. There were five different concentrations (0.00156%, 0.003125%, 0.00625%, 0.0125%, 0.025%) in each group of riboflavin. The fibroblasts were treated with riboflavin and the cellular viability was assessed at 24-hour and 48-hour post treatment with MTT 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide colorimetric assay. The absorbance values were analysed using Magellan microplate reader data analysis. A triplicate of readings was taken. The data were presented as mean ± standard deviation of the triplicates. Statistical analysis was performed with Statistical Package for Social Sciences (SPSS) analysis version 23. Irradiated riboflavin caused a concentration-dependent cell death in human tenon fibroblast cell culture ( p < .05). The antiproliferative difference between irradiated and non-irradiated riboflavin was significant up to 48 hours ( p < .05). Post hoc multiple comparisons showed higher concentrations of irradiated riboflavin (0.0125% and 0.025%) caused more reduction in cellular viability in human tenon fibroblast cells ( p < .05). The duration of treatment is not a causative factor in this study. This pilot experiment demonstrated that irradiated riboflavin induced cell death in human tenon fibroblast culture in a concentration-dependent manner, but is not time-dependent. Further exploratory investigations should be performed to determine the mechanism of cell death. We postulate that apoptosis occurred in these irradiated riboflavin-treated cells. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/The_Effect_of_Irradiated_Riboflavin_in_Human_Tenon_s_Fibroblast_A_Study_on_Cellular_Viability/17702158Test |
| DOI: |
10.6084/m9.figshare.17702158.v1 |
| الإتاحة: |
https://doi.org/10.6084/m9.figshare.17702158.v1Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.ACD552BB |
| قاعدة البيانات: |
BASE |
